بررسی نقش CREBI در مسیر سیگنالی هیدروکسی اوره / القای گاما گلوبین در سلولهای K552
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[پایاننامه]
Parallel Title Proper
Investigating the role of CREB1 in HU/globin induction signaling pathway in K562 cells
.PUBLICATION, DISTRIBUTION, ETC
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علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
Date of Publication, Distribution, etc.
، ۱۳۹۰
PHYSICAL DESCRIPTION
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۷۷ص.
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چاپی
DISSERTATION (THESIS) NOTE
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کارشناسی ارشد
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ژنتیک genetic
Date of degree
۱۳۹۰/۱۲/۰۷
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علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
SUMMARY OR ABSTRACT
Text of Note
thalassemia , HU , CREB1 , western blot , Dual luciferase assay-ع :promoter CREB1 response element (G-CRE) in this Pathway by using reporter assays. Since HU did not increase luciferase activity from our constructs, results of these experiments were not informative.Key words عglobin induction pathway.We also investigated role of a G- ع /expression through a Creb1/ATF-2 binding site present exclusively in the promoter of G We have used western blot to investigate levels of CREB1 in knockdown cells.Western blot analyses demonstrated that CREB1 becomes phosphorylated in a dose-dependent manner after HU treatment of K562 cells. These results suggest that Creb1 is required for the HU عvia p38 MAPK phosphorylation. It has been shown that SB induces G عinduction in erythroid cells. In addition, both HU and SB activate G (ع(and not A عglobin induction isnot completely understood. Similarities between the HU and sodium butyrate(SB) pathways suggested CREB1 as a potential candidate. The aim of this study was to investigate the possible role of CREB1 in this pathway. First, both HU and SB lead to G-ع globin transcription and fetal hemoglobin (HbF) production in erythroid cells. However its signaling pathway leading to-ع thalassemia treatment. HU functions by up-regulating-ع Hydroxyurea is commonly used for
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PARALLEL TITLE PROPER
Parallel Title
Investigating the role of CREB1 in HU/globin induction signaling pathway in K562 cells