عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
IR
۶۵۰پ
فارسی
IR
بررسی نقش CREBI در مسیر سیگنالی هیدروکسی اوره / القای گاما گلوبین در سلولهای K552
[پایان‌نامه]
Investigating the role of CREB1 in HU/globin induction signaling pathway in K562 cells
علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
، ۱۳۹۰
۷۷ص.
چاپی
کارشناسی ارشد
ژنتیک genetic
۱۳۹۰/۱۲/۰۷
علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
thalassemia , HU , CREB1 , western blot , Dual luciferase assay-ع :promoter CREB1 response element (G-CRE) in this Pathway by using reporter assays. Since HU did not increase luciferase activity from our constructs, results of these experiments were not informative.Key words عglobin induction pathway.We also investigated role of a G- ع /expression through a Creb1/ATF-2 binding site present exclusively in the promoter of G We have used western blot to investigate levels of CREB1 in knockdown cells.Western blot analyses demonstrated that CREB1 becomes phosphorylated in a dose-dependent manner after HU treatment of K562 cells. These results suggest that Creb1 is required for the HU عvia p38 MAPK phosphorylation. It has been shown that SB induces G عinduction in erythroid cells. In addition, both HU and SB activate G (ع(and not A عglobin induction isnot completely understood. Similarities between the HU and sodium butyrate(SB) pathways suggested CREB1 as a potential candidate. The aim of this study was to investigate the possible role of CREB1 in this pathway. First, both HU and SB lead to G-ع globin transcription and fetal hemoglobin (HbF) production in erythroid cells. However its signaling pathway leading to-ع thalassemia treatment. HU functions by up-regulating-ع Hydroxyurea is commonly used for
ba
Investigating the role of CREB1 in HU/globin induction signaling pathway in K562 cells
B-thalassemia
B-thalassemia
B-thalassemia
نظامی، مجید
Nezami, Majid
بنان، مهدی
نجم آبادی، حسین
ایران
دانشگاه علوم توان بخشی و سلامت اجتماعی
650..PDF
محرمانه
محرمانه
6501.PDF
6841213
مرجع
متن
0
۰۵۶پایا
محل نگهداری: دانشگاه علوم بهزیستی و توانبخشی
نمایه‌سازی قبلی
چاپی-الکترونیکی
TF
1
a
Y
