Stable isotope labeling by amino acids in cell culture (SILAC)
General Material Designation
[Book]
Other Title Information
methods and protocols /
First Statement of Responsibility
edited by Bettina Warscheid, Institute of Biology II, University of Freiburg, Germany.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York, NY :
Name of Publisher, Distributor, etc.
Humana Press,
Date of Publication, Distribution, etc.
[2014]
SERIES
Series Title
Methods in Molecular Biology,
Volume Designation
1188
ISSN of Series
1940-6029 ;
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
SUMMARY OR ABSTRACT
Text of Note
"Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--