عنوان

Stable isotope labeling by amino acids in cell culture (SILAC)

1493911414

9781493911417

9781493911431

9781493911424

b398306

Stable isotope labeling by amino acids in cell culture (SILAC)

[Book]

methods and protocols /

edited by Bettina Warscheid, Institute of Biology II, University of Freiburg, Germany.

New York, NY :

Humana Press,

[2014]

Methods in Molecular Biology,

1188

1940-6029 ;

Includes bibliographical references and index.

"Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--

Amino acids, Laboratory manuals.

Cell culture, Laboratory manuals.

Mass spectrometry.

Proteomics, Laboratory manuals.

Stable isotopes, Laboratory manuals.

Warscheid, Bettina.

20181223161536.0

[Book]

Y