edited by Jeffrey L. Bose, University of Kansas Medical Center, Kansas City, KS, USA
New York
Springer
2016
x, 186 pages : illustrations
Springer protocols
Methods in molecular biology,1064-3745 ;1373
Series: Springer protocols )Series(,1949-2448
Series: Methods in molecular biology )Clifton, N.J.( ;v. 1373,1064-3745
Understanding staphylococcal nomenclature /Christina N. Krute and Jeffrey L. Bose --Restriction-modification systems as a barrier for genetic manipulation of Staphylococcus aureus /Marat R. Sadykov --Complementation plasmids, inducible gene-expression systems, and reporters for Staphylococci /Ralph Bertram --De novo assembly of plasmids using yeast recombinational cloning /Ameya A. Mashruwala and Jeffrey M. Boyd --Splicing by overlap extension PCR to obtain hybrid DNA products /Justin A. Thornton --Method for preparation and electroporation of S. aureus and S. epidermidis /Melinda R. Grosser and Anthony R. Richardson --Rapid isolation of DNA from staphylococcus /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus : broth-based method /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus /Michael E. Olson --Batch transduction of transposon mutant libraries for rapid phenotype screening in staphylococcus /Katherine L. Maliszewski --Conjugative transfer in Staphylococcus aureus /Cortney R. Halsey and Paul D. Fey --Allelic exchange /McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles --Creation of staphylococcal mutant libraries using transposon Tn917 /Kelly C. Rice --Generation of a transposon mutant library in Staphylococcus aureus and staphylococcus epidermidis using bursa aurealis /Vijaya Kumar Yajjala ]and others[ --Chemical and UV mutagenesis /Jeffrey L. Bose --Pulse field gel electrophoresis /Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler Jr. --RNA-sequencing of Staphylococcus aureus messenger RNA /Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw --Quantitative real-time PCR )qPCR( workflow for analyzing Staphylococcus aureus gene expression /April M. Lewis and Kelly C. Rice --Electrophoretic mobility shiftaAssays /Sarah E. Rowe and James P. O'Gara --Rapid amplification of cDNA ends for RNA transcript sequencing in staphylococcus /Eric Miller.