عنوان

The genetic manipulation of staphylococci

(Number (ISBN

9781493931576 )alk. paper(

Title Proper

The genetic manipulation of staphylococci

First Statement of Responsibility

edited by Jeffrey L. Bose, University of Kansas Medical Center, Kansas City, KS, USA

Place of Publication, Distribution, etc.

New York

Name of Publisher, Distributor, etc.

Springer

Date of Publication, Distribution, etc.

2016

Specific Material Designation and Extent of Item

x, 186 pages : illustrations

Series Title

Springer protocols

Series Title

Methods in molecular biology,1064-3745 ;1373

Series Title

Series: Springer protocols )Series(,1949-2448

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1373,1064-3745

Text of Note

Understanding staphylococcal nomenclature /Christina N. Krute and Jeffrey L. Bose --Restriction-modification systems as a barrier for genetic manipulation of Staphylococcus aureus /Marat R. Sadykov --Complementation plasmids, inducible gene-expression systems, and reporters for Staphylococci /Ralph Bertram --De novo assembly of plasmids using yeast recombinational cloning /Ameya A. Mashruwala and Jeffrey M. Boyd --Splicing by overlap extension PCR to obtain hybrid DNA products /Justin A. Thornton --Method for preparation and electroporation of S. aureus and S. epidermidis /Melinda R. Grosser and Anthony R. Richardson --Rapid isolation of DNA from staphylococcus /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus : broth-based method /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus /Michael E. Olson --Batch transduction of transposon mutant libraries for rapid phenotype screening in staphylococcus /Katherine L. Maliszewski --Conjugative transfer in Staphylococcus aureus /Cortney R. Halsey and Paul D. Fey --Allelic exchange /McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles --Creation of staphylococcal mutant libraries using transposon Tn917 /Kelly C. Rice --Generation of a transposon mutant library in Staphylococcus aureus and staphylococcus epidermidis using bursa aurealis /Vijaya Kumar Yajjala ]and others[ --Chemical and UV mutagenesis /Jeffrey L. Bose --Pulse field gel electrophoresis /Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler Jr. --RNA-sequencing of Staphylococcus aureus messenger RNA /Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw --Quantitative real-time PCR )qPCR( workflow for analyzing Staphylococcus aureus gene expression /April M. Lewis and Kelly C. Rice --Electrophoretic mobility shiftaAssays /Sarah E. Rowe and James P. O'Gara --Rapid amplification of cDNA ends for RNA transcript sequencing in staphylococcus /Eric Miller.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Staphylococcus^agenetics

Entry Element

Genetic Phenomena

Entry Element

Genetic Techniques

Entry Element

Laboratory Manuals

Class number

Classification Record Number

Book number

Bose, Jeffrey L.

English Book