شماره کتابشناسی ملی
شماره
۲۴۱پ
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
per
عنوان و نام پديدآور
عنوان اصلي
معرفی مواد گزینشگر مناسب برای انتقال ژن به گیاه دارویی نعناع فلفلی (.Mentha piperita L)
نام نخستين پديدآور
/شیرین بهرام پور
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
: کشاورزی
مشخصات ظاهری
نام خاص و کميت اثر
۱۱۱ص
يادداشت کلی
متن يادداشت
جدول، نمودار، عکس
یادداشتهای مربوط به نشر، بخش و غیره
متن يادداشت
چاپی
یادداشتهای مربوط به کتابنامه ، واژه نامه و نمایه های داخل اثر
متن يادداشت
واژه نامه بصورت زیرنویس
متن يادداشت
کتابنامه ص.: ۱۰۳-۱۱۱
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
کارشناسی ارشد
نظم درجات
مهندسی کشاورزی- بیوتکنولوژی
زمان اعطا مدرک
۱۳۸۵/۰۶/۲۵
کسي که مدرک را اعطا کرده
تبریز
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
.Efficiency of transformation methods is due to precise, simple and quick identification of transformed and nontransformed tissues. To determine a suitable selective system has an important role in success of plant transformation. In this research, the effect of streptomycin and kanamycin antibiotics, glyphosate herbicide and a toxic compound called phenyl mercuric acetate (PMA) as selective agents were studied. For different concentrations and plant samples including leaf, leaf disk, shoot and callus, separate experiments were done for each selective agent. Plant samples were provided from field, greenhouse and in vitro cultures. The experiments were analyzed in as a split plot in time based on completely randomized design. Moreover, for quantification of the variation due to the effect of the antibiotics on plant samples, chlorophyll a, b and carotenoid were measured. Analysis of variance (ANOVA) showed a difference significant at 0.01 level for different PMA concentrations during studied days on shoot, leaf disk and leaf explants of peppermint. Mean comparison results showed that 2 to 4 and 1.5 ppm concentrations would cause plant death, in shoot and leaf disk explants, respectively. This result was obtained in the concentration of 1.5 ppm during 10 days. ANOVA of other selective agents containing kanamycin , streptomycin and glyphosate showed significant interactions, as well. Kanamycin concentration 100 ppm in shoot and bud samples caused whiteness of apexes and lateral buds and finally, leaf death. The sterptomysin concentration of 250 ppm was the optimal dose. Glyphosate concentration of 10000 and 2000 ppm caused necrosis and death of shoot and leaf samples during 2-3 and 6-9 days, respectively. Influence of this agent on leaf disks had different pattern in the field and greenhouse conditions. Studying the effect of the selective agents on callus, showed that using callus to produce transgenic plants seemed not to be feasible, so other methods such as morphogenesis, organogenesis and direct embryogenesis may be more effective. Analysis of variance of different streptomycin concentrations was significant at 0.01 level for photosynthetic characters including chlorophyll a, b, total chlorophyll, chlorophyll ratio a/b and carotenoid. Changes in mean values of these treatments for all the pigments showed decreasing trend while to increasing the streptomycin concentration. Results of assessing photosynthetic pigments for kanamycin were similar to those for streptomycin
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
بهرام پور، شیرین
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
باغبان کهنه روز، بهرام، استاد راهنما
مستند نام اشخاص تاييد نشده
مطلبی آذر، علیرضا، استاد مشاور
مستند نام اشخاص تاييد نشده
زهتاب سلماسی، سعید، استاد مشاور
وضعیت فهرست نویسی
وضعیت فهرست نویسی
نمایه‌سازی قبلی
