شماره کتابشناسی ملی
شماره
۲۳۰۵۷پ
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
per
عنوان و نام پديدآور
عنوان اصلي
مطالعه بیان ژنAS۱ - lnc TMPOدر سرطان معده
عنوان اصلي به زبان ديگر
Study of lnc TMPO_AS۱ gene expression in gastric cancer
نام نخستين پديدآور
/الهه محمدعلی
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
: علوم طبیعی
تاریخ نشرو بخش و غیره
، ۱۳۹۸
نام توليد کننده
، میرزائی
مشخصات ظاهری
نام خاص و کميت اثر
۶۳ص
یادداشتهای مربوط به نشر، بخش و غیره
متن يادداشت
چاپی - الکترونیکی
یادداشتهای مربوط به مندرجات
متن يادداشت
چکیده فارسی ایراد داشت.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
کارشناسی ارشد
نظم درجات
زیست شناسی سلولی و مولکولی گرایش ژنتیک
زمان اعطا مدرک
۱۳۹۸/۱۱/۱۹
کسي که مدرک را اعطا کرده
تبریز
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Gastric cancer is one of the most common cause of cancer-related death; besides, the diagnosis and therapy of it are still challenging. The chances of curing gastric cancer are greater if the cancer is diagnosed early. Researches have revealed that long non-coding RNAs (lncRNAs) play important roles in the initiation and progression of various human cancers, including gastric cancer (GC). Previous studies demonstrated a link between the changes of several lncRNAs expression level and onset and progression of GC. Study of lncRNAs in gastric cancer can make new advances in finding novel molecular diagnostic and prognostic biomarkers. TMPO antisense RNA۱ (TMPO-AS۱) gene, located at on chromosome ۱۲. Investigations showed that TMPO-AS۱ may be a diagnostic and prognostic marker in multiple cancer types such as prostate cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, cervical cancer, breast cancer, osteosarcoma, and colorectal cancer. Therefore, we aimed to evaluate the role of TMPO-AS۱ in gastric cancer. Methods In the present study, solid tissue samples from ۴۰ gastric tumors, and ۴۰ margin noncancerous tissues were obtained from ۴۰ GC patients. The samples were quickly freezing in liquid nitrogen immediately after tissue sampling during surgery to prevent RNA degradation. Total RNA was extracted by TRIzol using the guanidinium-isothiocyanate method. Afterward, The extracted RNAs were reverse-transcribed to cDNA using TAKARA cDNA synthesis kit. Finally real-time pcr technique was performed by using primer pairs for TMPO-AS۱ and GAPDH as internal control gene. The TMPO-AS۱ expression levels in patient's tissues samples were determined by qRT-PCR analysis. Calculated data were statistically analyzed using SPSS ۲۵ and GraphPad۶. Results The outcome of this experiment showed that the relative expression of TMPO-AS۱ increased in tumor tissues of GC patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay showed that TMPO-AS۱ was upregulated in GC tissues compared with matched margin noncancerous tissues. (p<۰.۰۰۷۶) Conclusion As indicated by the result, TMPO-AS۱ was overexpressed in gastric tumor tissues compared with margin noncancerous tissues. Therefore, it can be concluded that TMPO-AS۱ may be a potential diagnostic and prognostic biomarker to be used as a therapeutic target for GC; besides there is need for further to more researches to confirm these findings
عنوان اصلی به زبان دیگر
عنوان اصلي به زبان ديگر
Study of lnc TMPO_AS۱ gene expression in gastric cancer
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
محمدعلی، الهه
مستند نام اشخاص تاييد نشده
Mohammadali, Elaheh
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
صفرعلیزاده، رضا، استاد راهنما
مستند نام اشخاص تاييد نشده
برادران، بهزاد، استاد راهنما
مستند نام اشخاص تاييد نشده
اسودی، تورج، استاد مشاور
دسترسی و محل الکترونیکی
يادداشت عمومي
سیاه و سفید
وضعیت فهرست نویسی
وضعیت فهرست نویسی
نمایه‌سازی قبلی
