عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304414656
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
A comparative study of the base pairing properties of oligodeoxynucleotides containing 5-substituted-2'-deoxyuridines
نام عام مواد
[Thesis]
نام نخستين پديدآور
M. Ahmadian
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Purdue University
تاریخ نشرو بخش و غیره
1997
مشخصات ظاهری
نام خاص و کميت اثر
185
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
Purdue University
امتياز متن
1997
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Two series of modified dodecamer oligonucleotides were synthesized. The first contained the -C=CCH2R linker at C5 of deoxyuridine at position 4 (T*) of 5-d(CGCT*AATTAGCG)-3 and the second contained the -SR linker. The goal of the study was to evaluate and compare these two types of side-chains for suitablity as tethers for linking reporter groups to oligonucleotides. Our primary concern was how these tethers would affect duplex stability. The modified nucleosides were synthesized by palladium mediated coupling reactions between the substituted alkyne and 5-(4,4-dimethoxytrityl)-5-iodo-2usd \sp\primeusd-deoxyuridine and between a disulfide and 5-chloromercurio-2-deoxyuridine. The C5 deoxyuridine side chains evaluated included Cusd\equivusdCCH3, Cusd\equivusdCCH2NHC(O)CH3, Cusd\equivusdCCH2N(CH3)\sb2, Cusd\equivusdCCH2NHC(O)C5H4N, Cusd\equivusdCCH2NHC(O)CH, SCH3, SC6H5, and SCH2CH2NHC(O)CH3. The nucleosides containing these substituents were incorporated into oligodeoxyribonucleosides by standard phosphoramidite methodology. Melting studies demonstrated that the sequence containing the Cusd\equivusdCCH3 side chain had the highest usdT\sb{m}usd (60.0C) in comparison to the control sequence usd(T\sb{m}usd 55.9C), and that any additional substituent on C3 of the propynyl group lowered the usdT\sb{m}usd relative to propynyl. Nevertheless, even the most destabilizing substituent, adamantylcarbamoyl, yielded an oligodeoxyribonucleotide that melted at a usdT\sb{m}usd (55.4C) within 0.5C of the control sequence. In contrast the thioether substituents led to lower usdT\sb{m}usd's ranging from 46.4C for SPh to 53.3C for SCH3. However, the oligonucleotide containing the thioether substituent, SCH2CH2NHC(O)CH3 usd(T\sb{m}usd = 52.5C) melted with higher cooperativity than any of the sequences tested and was shown to have the largest negative free energy of formation usd\rm (G\sb{25\sp\circ} = {-}14.1).usd
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Molecular biology
موضوع مستند نشده
Organic chemistry
موضوع مستند نشده
Pure sciences
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
M. Ahmadian
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
