عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304439660
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Turkey beta-adrenergic receptors:
نام عام مواد
[Thesis]
نام نخستين پديدآور
F. Del Toro, Jr.
عنوان اصلي به قلم نويسنده ديگر
Search for a mammalian homologue of a novel turkey receptor and desensitization of the turkey beta(2)-adrenergic receptor
نام ساير پديدآوران
R. A. Nicholas
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
The University of North Carolina at Chapel Hill
تاریخ نشرو بخش و غیره
1998
مشخصات ظاهری
نام خاص و کميت اثر
134-134 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
The University of North Carolina at Chapel Hill
امتياز متن
1998
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
The mammalian usd\betausd-AR family has three well-characterized subtypes, the usd\beta\sb1usd-, usd\beta\sb2usd-, and usd\beta\sb3usd-adrenergic receptors. Recent cloning of a novel turkey usd\betausd-adrenergic receptor (usd\betausd-AR), called the usd\beta\sb{\rm 4c}usd-AR, has suggested the possibility of the existence of an additional mammalian usd\betausd-AR. We utilized PCR, Southern blot analysis, and library screening in an effort to clone a potential human homologue of the turkey usd\beta\sb{\rm 4c}usd-AR. Screening of two human genomic libraries resulted in the isolation of 29 clones encoding human usd\betausd-ARs: 19 usd\beta\sb1usd-AR clones, 3 usd\beta\sb2usd-AR clones, and 7 usd\beta\sb3usd-AR clones. In addition, two clones encoding the 5HT1a receptor were isolated. Since all of the known usd\betausd-AR genes were cloned by this method, but no usd\beta\sb{\rm 4c}usd-AR homologue, these studies suggest that a mammalian homologue of the turkey usd\beta\sb{\rm 4c}usd-AR does not exist. Turkeys also express a usd\beta\sb2usd-AR with 82% identity to the human usd\beta\sb2usd-AR that terminates 16 aminoacids earlier than the human usd\beta\sb2usd-AR. Furthermore, the turkey usd\beta\sb2usd-AR lacks multiple serine and threonine residues in its carboxy-terminus, suggesting a potential alteration in the desensitization properties of the turkey receptor compared to human. We expressed both turkey and human usd\beta\sb2usd-ARs in the same cell line at similar receptor densities and showed that the affinities of a series of agonists and antagonists were nearly identical at both receptors. Functionally, both turkey and human usd\beta\sb2usd-ARs couple to elevations in cellular cyclic AMP and their propensities to undergo both homologous and long-term desensitization were nearly identical. These data indicate that the desensitization properties of the turkey and human usd\beta\sb2usd-adrenergic receptors are very similar, even though the turkey usd\beta\sb2usd-AR lacks multiple phosphorylation sites.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Adrenergic
موضوع مستند نشده
Beta adrenergic receptor
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Health and environmental sciences
موضوع مستند نشده
Pharmacology
موضوع مستند نشده
Turkey receptor
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
F. Del Toro, Jr.
مستند نام اشخاص تاييد نشده
R. A. Nicholas
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
