عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL58010
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Investigating the Physiological Role of Chloride Permeation through Excitatory Amino Acid Transporter 1 (EAAT1)
نام عام مواد
[Thesis]
نام نخستين پديدآور
Akhter, Muhammad Azman
نام ساير پديدآوران
van Meyel, Don
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
McGill University (Canada)
تاریخ نشرو بخش و غیره
2019
يادداشت کلی
متن يادداشت
68 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.Sc.
کسي که مدرک را اعطا کرده
McGill University (Canada)
امتياز متن
2019
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Astrocytes maintain ion and neurotransmitter homeostasis within the central nervous system. They do so by expressing channels, receptors and transporters such as excitatory amino acid transporters (EAATs) along their highly ramified arbours. EAATs are well known to be glutamate transporters, but they have a dual function as chloride channels. The conservation of the EAAT anion channel from GltPh in prokaryotes to mammals suggests an essential physiological role. However, few studies have addressed the role of chloride permeation through EAAT1 in vivo, so its importance for normal brain function is not yet completely understood. During my project, I built tools to separate the chloride channel and glutamate transport functions of the human EAAT1 (hEAAT1) protein. Through an extensive literature review, I identified a series of hEAAT1 mutations that only affect the permeation and gating properties of the Cl- channel, without affecting glutamate transport function. I built transgenic flies where these altered hEAAT1 proteins were expressed in Drosophila astrocytes in vivo. Additionally, I built transgenic flies to investigate the contribution of hEAAT1 Cl- channel in the cytopathology of Episodic Ataxia Type 6 using five new patient-derived mutations. Using an antibody to hEAAT1, I first validated the expression and proper localization of these altered hEAAT1 proteins within glial cells. Additionally, I developed an improved and optimized method to quantify the crawling behaviour of L1 larvae. With this locomotion assay, I showed that EAAT1 is required in astrocytes for motor behavior, and that ensheathing glia provide an EAAT1-mediated function that is essential for animal survival. Using these transgenic flies coupled with the new locomotion assay, our lab is poised to determine the physiological role of hEAAT1 Cl- channel in glial cells. Our preliminary data provide the first direct evidence that the hEAAT1 Cl- channel is essential for its function in glial cells in vivo under physiological conditions.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Amino acids
اصطلاح موضوعی
Chloride
اصطلاح موضوعی
Graduate students
اصطلاح موضوعی
Homeostasis
اصطلاح موضوعی
Insects
اصطلاح موضوعی
Mammals
اصطلاح موضوعی
Mutation
اصطلاح موضوعی
Nervous system
اصطلاح موضوعی
Physiology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Akhter, Muhammad Azman
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
van Meyel, Don
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
McGill University (Canada)
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
