عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL50445
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Generation and Characterization of Induced Pluripotent Stem Cells Derived from Parkinson's Disease Patients with Familial A53T Mutation and Sporadic Cases
نام عام مواد
[Thesis]
نام نخستين پديدآور
Swaidan, Nuha Taysir
نام ساير پديدآوران
Emara, Mohamed M.
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Hamad Bin Khalifa University (Qatar)
تاریخ نشرو بخش و غیره
2019
يادداشت کلی
متن يادداشت
87 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.S.
کسي که مدرک را اعطا کرده
Hamad Bin Khalifa University (Qatar)
امتياز متن
2019
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Nowadays, induced pluripotent stem cells (iPSCs) are the most highlighted type of stem cells in research as they overcome the limitations associated with embryonic stem cells (ESCs) usage and hold a promising future for disease modeling and treatment. Generation of iPSCs is dependent on reprogramming of somatic cells by the forced introduction of reprogramming factors into the cell. To date, reprogramming procedure remains inefficient process, since a small portion of transfected cells can undergo complete reprogramming. One of the identified factors that affect the reprogramming efficiency is donor age. In this thesis, we aim to test the effect of donor age on reprogramming process using skin fibroblasts collected from PD patients who were diagnosed with early onset familial PD (A53T mutation) and late onset sporadic PD. To achieve this goal, fibroblasts were reprogrammed using Sendai virus vector to generate iPSCs. All the generated iPSCs were compared to ESCs and the commercially available iPSCs to ensure that these generated iPSCs have the same characteristic features and to confirm their ability to express pluripotent protein markers. The number of emerged colonies was used to reflect the reprogramming efficiency. In our study, we observed that A53T (2) sample showed the highest number of iPSC colonies among the other reprogrammed samples. Our results showed that the donor age had no effect on our reprogramming procedure as number of emerged iPSC colonies in sporadic PD (age 69) was similar to both control and other A53T samples (age 45 and 48, respectively) that were isolated from individuals who were similar in age with A53T (2) sample. Therefore, further transcriptomic analysis is required to identify intrinsic factor(s) that may play a role in the significantly high number of iPSC colonies observed with A53T (2) reprogramming.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Cellular biology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Swaidan, Nuha Taysir
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Emara, Mohamed M.
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
Hamad Bin Khalifa University (Qatar)
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
