عنوان

RT-PCR protocols.

شابک

1607616289

شابک

1607616297

شابک

9781607616283

شابک

9781607616290

شماره

b794684

عنوان اصلي

RT-PCR protocols.

نام عام مواد

[Book]

وضعيت ويراست

2nd ed. /

نام نخستين ويراستار

edited by Nicola King.

محل نشرو پخش و غیره

New York :

نام ناشر، پخش کننده و غيره

Humana,

تاریخ نشرو بخش و غیره

©2010.

نام خاص و کميت اثر

1 online resource (xi, 341 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology,

عنوان فروست

Springer protocols

مشخصه جلد

630

شاپا ي ISSN فروست

1064-3745 ;

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Single cell RT-PCR on mouse embryos : a general approach for developmental biology / Wolfgang Mann and Thomas Haaf -- Poly(A) cDNA real-time PCR for indicator gene measurement in cancer / Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers -- Transcriptome profiling of host-microbe interactions by differential display RT-PCR / Leong Wai Fook and Vincent T.K. Chow -- Quantitative RT-PCR methods for mature microRNA expression analysis / Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson -- Detection of influenza A virus neuraminidase and PB2 gene segments by one step reverse transcription polymerase chain reaction / Alejandra Castillo Alvarez [and others] -- Detection and identification of CD46 splicing isoforms by nested RT-PCR / Anita Szalmás [and others] -- Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR / Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq -- Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR / Ana Silvia González-Reiche [and others] -- Detection of antisense RNA transcripts by strand-specific RT-PCR / Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville -- RT-PCR amplification and cloning of large viral sequences / Xiaofeng Fan and Adrian M. Di Bisceglie -- One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification / Cristina Hartshorn and Lawrence J. Wangh -- Changes in gene expression of caveolin-1 after inflammatory pain using quantitative real-time PCR / Fiza Rashid-Doubell -- Real-time quantitative reverse transcriptase polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye -- The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes / Nicola King -- The renaissance of competitive PCR as an accurate tool for precise nucleic acid quantification / Lorena Zentilin and Mauro Giacca -- Skeletal muscle RNA extraction in preparation for RT-PCR / Janelle P. Mollica -- Reverse transcription of the ribonucleic acid : the first step in RT-PCR assay / Fadia Haddad and Kenneth M. Baldwin -- Primer design for RT-PCR / Kelvin Li and Anushka Brownley -- Hot start PCR / Natasha Paul, Jonathan Shum, and Tony Le -- Real-time RT-PCR for automated detection of HIV-1 RNA during blood donor screening / Jens Müller.

بدون عنوان

0

متن يادداشت

Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

منبع سفارش / آدرس اشتراک

Springer

شماره انبار

978-1-60761-628-3

عنوان

RT-PCR protocols.

شماره استاندارد بين المللي کتاب و موسيقي

1607616289

موضوع مستند نشده

Polymerase chain reaction, Laboratory manuals.

موضوع مستند نشده

Reverse Transcriptase Polymerase Chain Reaction.

موضوع مستند نشده

Polymerase chain reaction.

موضوع مستند نشده

MED107000

موضوع مستند نشده

MFN

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

شماره رده

نشانه اثر

کد سيستم

مستند نام اشخاص تاييد نشده

King, Nicola,Dr.

تاريخ عمليات

20201207074830.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y