عنوان

A low-cost approach to PCR :

شابک

0195119266

شابک

0198028016

شابک

1280529784

شابک

1429401311

شابک

6610529787

شابک

9780195119268

شابک

9780198028017

شابک

9781280529788

شابک

9781429401319

شابک

9786610529780

شماره

b768472

عنوان اصلي

A low-cost approach to PCR :

نام عام مواد

[Book]

ساير اطلاعات عنواني

appropriate transfer of biomolecular techniques /

نام نخستين پديدآور

Eva Harris ; edited by Nazreen Kadir.

محل نشرو پخش و غیره

New York :

نام ناشر، پخش کننده و غيره

Oxford University Press,

تاریخ نشرو بخش و غیره

1998.

نام خاص و کميت اثر

1 online resource (xxi, 304 pages) :

ساير جزييات

illustrations

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

1 Introduction -- 2 PCR Technology -- 2.1. Description of the Technique -- 2.1.1. Historical Overview -- 2.1.2. The Molecular Basis of PCR -- 2.1.3. Advantages and Disadvantages -- 2.2. Technical Details -- 2.2.1. Components of the PCR Mixture -- 2.2.2. Thermal Cycling Parameters -- 2.2.3. Optimizing -- 2.2.4. Potential Problems -- 2.2.5. Detection of Products -- 2.3. Frequently-Used PCR-Based Techniques -- 3 Principles of Sustainable Technology Transfer -- 3.1. A Low-Cost Methodology -- 3.1.1. Appropriate Technology -- 3.1.2. Alternative Techniques -- 3.1.3. Simplification of Protocols -- 3.1.4. In-House Preparation of Reagents -- 3.1.5. Recycling -- 3.1.6. Donated Materials -- 3.2. Knowledge-Based Participatory Transfer Process -- 3.3. Appropriate Application -- 3.3.1. General Considerations -- 3.3.2. An Evaluation Framework -- 3.3.3. Assessment Criteria for PCR -- 3.3.4. Case-by-Case Evaluation of PCR Applied to Infectious Diseases -- 3.4. Intra-Reginal Cooperation.

متن يادداشت

4 Case Study: The AMB/ATT Program -- 4.1. Introduction -- 4.2. Program Description -- 4.2.1. Objectives -- 4.2.2. Format -- 4.3. Program Structure -- 4.3.1. Phase -- 4.3.2. Phase -- 4.3.3. Phase -- 4.4. Program Development -- 4.5. Examples of Projects -- 4.6. International Courses -- II Selected Protocols -- 5.1. PCR Protocols -- 5.1.1. Overview -- 5.1.2. Equipment, Materials, Control, and Procedures Common to All PCR Protocols -- 5.1.3. Dengue Virus -- 5.1.4. New World Leishmania -- 5.1.5. Mycobactrium tuberculosis -- 5.1.6. Plasmodium falciparum and Plasmodium vivax -- 5.1.7. Vibrio cholerae -- 5.1.8. Diarrheagenic E. coli and Shigella -- 5.1.9. Chlamydia trachomatis and Neisseria gonorrhoeae -- 5.1.10. Leptospira -- 5.1.11. Trypanosoma cruzi -- 5.2. Nonradioactive DNA Probes: V. cholerae Colony Blot -- 5.2.1. Labeling the Probe Using PCR -- 5.2.2. Preparation of the Colony Blot -- 5.2.3. Hybridization -- 5.2.4. Visualization -- 6 Rapid Cloning of PCR Products -- 6.1. Primer Design.

متن يادداشت

6.2. Preparation of PCR Products for Cloning -- 6.2.1. Digestion of Vector and PCR Products -- 6.2.2. Preparative Agarose Gel -- 6.2.3. Purification of the Excised DNA Fragments Using Silica Particles -- 6.3. Ligation -- 6.3.1. Checking DNA Fragment Concentration by Agarose Gel Electrophoresis -- 6.3.2. Ligation Reaction -- 6.4. Preparation of Competent Cells and Transformation -- 6.4.1. Preparation of Competent Cells -- 6.4.2. Transformation -- 6.5. Checking Clones by PCR -- 6.6. Plasmid Purification -- 6.7. Analysis of Clones by Restriction Enzyme Digestion -- 6.8. Agarose Gel Electrophoresis -- III Appendix -- A. Construction of Laboratory Equipment -- B. In-House Preparation of Reagents -- B.1 Useful Formulas -- B.2 Solutions -- B.3 Preparation of Selected Reagents -- B.4 DNA Size Markers -- C. Inventory for a PCR Laboratory -- D. Good Laboratory Practice -- D.1 General Tips -- D.2 Calibration of Adjustable Pipettors -- E. Prevention of Cross-Contamination -- F. PCR Troubleshooting Guide and Flow-Chart -- G. Workshop Organization and Teaching Tips -- H. Sample Charts and Worksheets -- I. Useful World Wide Web Sites.

بدون عنوان

0

بدون عنوان

0

بدون عنوان

0

متن يادداشت

The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad.; In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.

عنوان

Low-cost approach to PCR.

موضوع مستند نشده

Molecular biology-- Technology transfer.

موضوع مستند نشده

Polymerase chain reaction, Laboratory manuals.

موضوع مستند نشده

Molecular Biology.

موضوع مستند نشده

Polymerase Chain Reaction.

موضوع مستند نشده

Biologia molecular.

موضوع مستند نشده

Methode

موضوع مستند نشده

Molecular biology-- Technology transfer.

موضوع مستند نشده

Polymerase chain reaction.

موضوع مستند نشده

Polymerase-Kettenreaktion

موضوع مستند نشده

Reaccion en cadena de la polimerasa.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Molecular Biology.

موضوع مستند نشده

Transferencia de tecnologia.

موضوع مستند نشده

Vereinfachung

موضوع مستند نشده

PDN

موضوع مستند نشده

SCI-- 049000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

نشانه اثر

کد سيستم

کد سيستم

کد سيستم

کد سيستم

مستند نام اشخاص تاييد نشده

Harris, Eva,1965-

مستند نام اشخاص تاييد نشده

Kadir, Nazreen.

تاريخ عمليات

20201206140650.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y