عنوان

RNA :

شابک

1597452483

شابک

9781597452489

شابک اشتباه

1588299139

شابک اشتباه

9781588299130

شماره

b708703

عنوان اصلي

RNA :

نام عام مواد

[Book]

ساير اطلاعات عنواني

methods and protocols /

نام نخستين پديدآور

edited by Henrik Nielsen.

محل نشرو پخش و غیره

New York, N.Y. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2011.

نام خاص و کميت اثر

1 online resource (xv, 329 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology,

عنوان فروست

Springer Protocols.

مشخصه جلد

703

شاپا ي ISSN فروست

1940-6029 ;

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

The transcriptional landscape -- Working with RNA -- Synthesis of RNA by in vitro transcription -- Efficient poly(A)+ RNA selection using LNA oligo(T) capture -- Genome browsers -- Web-based tools for studying RNA structure and function -- Northern blotting analysis -- Rapid amplification of cDNA ends (RACE) -- Fractionation of mRNA based on the length of the poly(A) tail -- Analysis of mutations that influence pre-mRNA splicing -- S1 nuclease analysis of alternatively spliced mRNA -- Promises and challenges in developing RNAi as a research tool and therapy -- Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) -- Validation of RNAi by real time PCR -- Profiling RNA polymerase II using the fast chromatin immunoprecipitation method -- The post-transcriptional operon -- RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling -- Isolation of RNP granules -- Electrophoretic mobility shift assay for characterizing RNA-protein interaction -- Polysome analysis and RNA purification from sucrose gradients -- Prediction of targets for microRNAs -- Outsourcing of experimental work.

بدون عنوان

0

متن يادداشت

Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of "gene X" and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology. series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.

منبع سفارش / آدرس اشتراک

Springer

شماره انبار

978-1-58829-913-0

عنوان

RNA.

شماره استاندارد بين المللي کتاب و موسيقي

9781588299130

موضوع مستند نشده

Messenger RNA.

موضوع مستند نشده

Non-coding RNA.

موضوع مستند نشده

RNA, Messenger.

موضوع مستند نشده

RNA, Untranslated.

موضوع مستند نشده

Messenger RNA.

موضوع مستند نشده

Non-coding RNA.

موضوع مستند نشده

MED107000

موضوع مستند نشده

MFN

شماره

ويراست

شماره رده

شماره رده

مستند نام اشخاص تاييد نشده

Nielsen, Henrik.

تاريخ عمليات

20201207233828.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y