عنوان

Expanding the bioluminescent toolkit for in vivo imaging

شماره

TL5dt6g5pz

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Expanding the bioluminescent toolkit for in vivo imaging

نام عام مواد

[Thesis]

نام نخستين پديدآور

Paley, Miranda Amelia

نام ساير پديدآوران

Prescher, Jennifer A

تاریخ نشرو بخش و غیره

2014

کسي که مدرک را اعطا کرده

Prescher, Jennifer A

امتياز متن

2014

متن يادداشت

Bioluminescence imaging (BLI) is among the most dynamic imaging modalities for visualizing whole cells and gene expression patterns in vivo. This technique captures light emission from the luciferase-catalyzed oxidation of small molecule luciferins with highly sensitive CCD cameras. While powerful, current options for multiplexed BLI in mice are limited by the number of luciferase/luciferin pairs found in nature. Our lab aims to expand the bioluminescent toolkit by pairing mutant luciferases with synthetic luciferin analogs, to biochemically resolve multiple targets via sequential administration of the substrates. Several generations of luciferase mutant libraries were screened against sterically and electronically modified families of luciferins, in order to find orthogonal pairs. Promising luciferases were expressed recombinantly for biochemical analysis with their respective luciferins. These pairs were evaluated for their potential for multicomponent in vivo imaging.

مستند نام اشخاص تاييد نشده

Reyes de Mochel, Nabora Soledad

مستند نام اشخاص تاييد نشده

Paley, Miranda Amelia

مستند نام تنالگان تاييد نشده

UC Irvine

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y