عنوان

Transgenesis techniques :

شابک

0896032450 (pbk.)

شابک

9780896032453 (pbk.)

شماره

b520532

عنوان اصلي

Transgenesis techniques :

نام عام مواد

[Book]

ساير اطلاعات عنواني

principles and protocols /

نام نخستين پديدآور

edited by David Murphy, David A. Carter.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

[1993]

تاریخ نشرو بخش و غیره

©1993

نام خاص و کميت اثر

xii, 467 pages :

ساير جزييات

illustrations ;

ابعاد

23 cm.

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 18

متن يادداشت

Includes bibliographical references and index

متن يادداشت

(cont) Genomic analysis of transgenic animals: southern blotting -- Expression analysis of transgenic animals -- Preparation of RNa from the tissues of transgenic animals -- Northern blotting -- RNA slot blotting -- Analysis of gene expression by PCR -- Nuclear run-on analysis of transcription -- S1 nuclease protection mapping -- Primer extension -- In situ hybridization analysis of transgenic tissues -- Immunohistochemical analysis of transgene expression -- Postmortem examination of transgenic mice -- Histological examination of transgenic mice -- Reporter enzyme assays -- Appendix -- Restriction endonuclease digestion of DNA -- Agarose gel electrophoresis -- Working with radioactivity in the transgenic animal laboratory -- Random primed labeling of DNA -- Filter hybridization -- DNA transfection

متن يادداشت

Introduction and topical reviews -- Introduction to transgenesis -- Transgenic rodents and the study of the central nervous system -- Transgenic animals and the study of cancer -- Transgenic animals and the study of the immune system -- Commercial and agricultural applications of animal transgenesis -- Transgenesis in invertebrate and lower vertebrate species -- P element-mediated germ-line transformation of Drosophila -- Gene transfer by microinjection in the zebra fish Brachydanio rerio -- Transgenic induction in Salmonid and Tilapia fish -- Transgenesis in the mouse -- Overview of transgenic mouse production -- Transgene design -- Isolation of DNA fragments for microinjection -- Establishing a colony for efficient production of transgenic mice -- Mating mice -- Anesthetizing mice -- Vasectomizing a mouse -- Preparation of culture media for fertilized one-cell mouse eggs -- Collection of fertilized one-cell mouse eggs for microinjection

متن يادداشت

Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs I: manual injection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs II: automatic injection -- Delivery of microinjected eggs to surrogate mothers by oviduct transfer -- Caesarean section and fostering -- In vitro fertilization of mouse eggs -- In vitro isolation of murine embryonic stem cells -- Production of chimeras derived from murine embryonic stem cells -- Cryopreservation of transgenic mouse lines -- Transgenesis in the rat -- Introduction to the physiology and husbandry of the rat -- Mating of rats -- Vasectomy of the male rat -- Harvest of fertilized one-cell rat eggs -- Implantation of microinjected eggs -- Cryopreservation of transgenic rat lines -- Transgenesis in a domestic species: the sheep -- Production of transgenic sheep -- Cryopreservation of transgenic sheep lines -- Genomic analysis of transgenic animals -- Isolation of genomic DNA from tail tissue -- Slot blotting of genomic tail DNA -- Genomic analysis of transgenic animals by the polymerase chain reaction

بدون عنوان

0

بدون عنوان

0

بدون عنوان

0

متن يادداشت

Mode of access: World Wide Web

عنوان

Transgenesis techniques : principles and protocols

عنوان

OhioLINK electronic book center

عنوان

SpringerLink

موضوع مستند نشده

Animal genetic engineering-- Technique.

موضوع مستند نشده

Genetic transformation-- Technique.

موضوع مستند نشده

Transgenic animals.

مستند نام اشخاص تاييد نشده

Carter, David A., (David Allan),1954-

مستند نام اشخاص تاييد نشده

Murphy, David,1957-

مستند نام تنالگان تاييد نشده

Ohio Library and Information Network.

تاريخ عمليات

20081117041421.0

قواعد فهرست نويسي ( بخش توصيفي )

rda

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y