عنوان

The protein protocols handbook /

شابک

0896039404

شابک

0896039412

شابک

1592591698

شابک

9780896039407

شابک

9780896039414

شابک

9781592591695

شماره

dltt

عنوان اصلي

The protein protocols handbook /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by John M. Walker

وضعيت ويراست

Second edition

نام خاص و کميت اثر

xxiv, 1,146 pages :

ساير جزييات

illustrations ;

ابعاد

26 cm

متن يادداشت

Includes bibliographical references and index

متن يادداشت

Protein Determination by UV Absorption -- The Lowry Method for Protein Quantitation -- The Bicinchoninic Acid (BCA) Assay tor Protein Quantitation -- The Bradford Method tor Protein Quantitation -- Ultrafast Protein Determinations Using Microwave Enhancement -- The Nitric Acid Method tor Protein Estimation in Biological Samples -- Quantitation of Tryptophan in Proteins -- Flow Cytometric Quantitation of Cellular Proteins -- Kinetic Silver Staining of Proteins -- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins -- SDS Polyacrylamide Gel Electrophoresis of Proteins -- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins -- SDS-Polyacrylamide Gel Electrophoresis of Peptides -- Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-Page) -- Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M -Based Separation of Proteins with Retained Native Activity -- Acetic-Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins -- Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones -- lsoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels -- Protein Solubility in Two-Dimensional Electrophoresis: Basic Principles and Issues -- Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis -- Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-D Electrophoresis -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Carrier Ampholyte pH Gradients in the First Dimension -- Casting Immobilized pH Gradients (IPGs) -- Nonequilibrium pH Gel Electrophoresis (NEPHGE) -- Difference Gel Electrophoresis -- Comparing 2-D Electrophoretic Gels Across Internet Databases -- lmmunoblotting of 2-D Electrophoresis Separated Proteins -- Quantification of Radiolabeled Proteins in Polyacrylamide Gels -- Quantification of Proteins on Polyacrylamide Gels -- Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red -- Zinc-Reverse Staining Technique -- Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels -- Detection of Proteins in Polyacrylamide Gels by Silver Staining -- Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains -- Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining -- Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain -- Electroelution of Proteins from Polyacrylamide Gels -- Autoradiography and Fluorography of Acrylamide Gels -- Protein Blotting by Electroblotting -- Protein Blotting by the Semidry Method -- Protein Blotting by the Capillary Method -- Protein Blotting of Basic Proteins Resolved on Acid-Urea-Trinton-Polyacrylamide Gels -- Alkaline Phosphatase Labeling of lgG Antibody -- (Beta)-Galactosidase Labeling of lgG Antibody -- Horseradish Peroxidase Labeling of lgG Antibody -- Digoxigenin (DIG) Labeling of lgG Antibody -- Conjugation of Fluorochromes to Antibodies -- Coupling of Antibodies with Biotin -- Preparation of Avidin Conjugates -- MDPF Staining of Proteins on Western Blots -- Copper Iodide Staining of Proteins and Its Silver Enhancement -- Detection of Proteins on Blots Using Direct Blue 71 -- Protein Staining and lmmunodetection Using lmmunogold -- Detection of Polypeptides on lmmunoblots Using Enzyme-Conjugated or Radiolabeled Secondary Ligands -- Utilization of Avidin- or Streptavidin-Biotin as a Highly Sensitive Method to Stain Total Proteins on Membranes -- Detection of Protein on Western Blots Using Chemifluorescence -- Quantification of Proteins on Western Blots using ECL -- Reutilization of Western Blots After Chemiluminescent Detection or Autoradiography -- Carboxymethylation of Cysteine Using lodoacetamide/lodoacetic Acid -- Performic Acid Oxidation -- Succinylation of Proteins -- Pyridylethylation of Cysteine Residues -- Side Chain Selective Chemical Modifications of Proteins -- Nitration of Tyrosines -- Ethoxyformylation of Histidine -- Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal -- Amidation of Carboxyl Groups -- Amidination of Lysine Side Chains -- Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide -- Modification of Sulfhydryl Groups with DTNB -- Chemical Cleavage of Proteins at Methionyi-X Peptide Bonds -- Chemical Cleavage of Proteins at Tryptophanyi-X Peptide Bonds -- Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds -- Chemical Cleavage of Proteins at Cysteinyi-X Peptide Bonds -- Chemical Cleavage of Proteins at Asparaginyi-Giycyl Peptide Bonds -- Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels -- Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis -- Reverse Phase HPLC Separation of Enzymatic Digests of Proteins -- Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography -- Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis -- Peptide Mapping by High-Performance Liquid Chromatography -- Production of Protein Hydrolysates Using Enzymes -- Amino Acid Analysis by Precolumn Derivatization with 1-Fiuoro-2,4- Dinitrophenyi-5-L -Alanine Amide (Marley's Reagent) -- Molecular Weight Estimation for Native Proteins Using High-Performance Size-Exclusion Chromatography -- Detection of Disulfide-Linked Peptides by HPLC -- Detection of Disulfide-Linked Peptides by Mass Spectrometry -- Diagonal Electrophoresis tor Detecting Disulfide Bridges -- Estimation of Disulfide Bonds Using Ellman's Reagent -- Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis -- Analyzing Protein Phosphorylation -- Mass Spectrometric Analysis of Protein Phosphorylation -- Identification of Proteins Modified by Protein (o-Aspartyi/L -lsoaspartyl) Carboxyl Methyltransferase

متن يادداشت

Analysis of Protein Palmitoylation -- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins: A Useful Tool for Studying Protein Prenylation -- The Metabolic Labeling and Analysis of lsoprenylated Proteins -- 2-D Phosphopeptide Mapping -- Detection and Characterization of Protein Mutations by Mass Spectrometry -- Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry -- Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Protein Identification Using Peptide and Fragmention Masses -- Protein Ladder Sequencing -- Sequence Analysis with WinGene/WinPep -- Isolation of Proteins Cross-linked to DNA by Cisplatin -- Isolation of Proteins Cross-linked to DNA by Formaldehyde -- Detection of Glycoproteins in Gels and Blots -- Staining of Glycoproteins/Proteoglycans in SDS-Gels -- Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting -- A Lectin-Binding Assay tor the Rapid Characterization of the Glycosylation of Purified Glycoproteins -- Chemical Methods of Analysis of Glycoproteins -- Monosaccharide Analysis by HPAEC -- Monosaccharide Analysis by Gas Chromatography (GC) -- Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis -- Sialic Acid Analysis by HPAEC-PAD -- Chemical Release of O-Linked Oligosaccharide Chains -- O-Linked Oligosaccharide Profiling by HPLC -- O -Linked Oligosaccharide Profiling by HPAEC-PAD -- Release of N-Linked Oligosaccharide Chains by Hydrazinolysis -- Enzymatic Release of O- and N-Linked Oligosaccharide Chains -- N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC) -- N-Linked Oligosaccharide Profiling by HPAEC-PAD -- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the CarboPac MA 1 Column -- Microassay Analyses of Protein Glycosylation -- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins -- HPLC Analysis of Fluorescently Labeled Glycans -- Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance -- Sequencing Heparan Sulfate Saccharides -- Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis and Mass Spectrometry -- Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins -- Antibody Production -- Production of Antibodies Using Proteins in Gel Bands -- Raising Highly Specific Polyclonal Antibodies Using Biocompatible Support-Bound Antigens -- Production of Antisera Using Peptide Conjugates -- The Chloramine T Method for Radiolabeling Protein -- The Lactoperoxidase Method for Radiolabeling Protein -- The Bolton and Hunter Method for Radiolabeling Protein -- Preparation of 1251 Labeled Peptides and Proteins with High Specific Activity Using IODO-GEN -- Purification and Assessment of Quality of Radioiodinated Protein -- Purification of lgG by Precipitation with Sodium Sulfate or Ammonium Sulfate -- Purification of lgG Using Caprylic Acid -- Purification of lgG Using DEAE-Sepharose Chromatography -- Purification of lgG Using ion-Exchange HPLC -- Purification of lgG by Precipitation with Polyethylene Glycol (PEG) -- Purification of lgG Using Protein A or Protein G -- Analysis and Purification of lgG Using Size-Exclusion High Performance Liquid Chromatography (SE-HPLC) -- Purification of lgG Using Affinity Chromatography on Antigen-Ligand Columns -- Purification of lgG Using Thiophilic Chromatography -- Analysis of lgG Fractions by Electrophoresis -- Purification of Immunoglobulin Y (lgY) from Chicken Eggs -- Affinity Purification of Immunoglobulins Using Protein A Mimetic (PAM) -- Detection of Serological Cross-Reactions by Western Cross-Blotting -- Bacterial Expression, Purification, and Characterization of Single-Chain Antibodies -- Enzymatic Digestion of Monoclonal Antibodies -- How to Make Bispecific Antibodies -- Phage Display: Biopanning on Purified Proteins and Proteins Expressed in Whole Cell Membranes -- Screening of Phage Displayed Antibody Libraries -- Antigen Measurements Using ELISA -- Enhanced Chemiluminescence Immunoassay -- lmmunoprecipitation -- Immunogen Preparation and Immunization Procedures for Rats and Mice -- Hybridoma Production -- Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay -- Screening Hybridoma Culture Supernatants Using ELISA -- Growth and Purification of Murine Monoclonal Antibodies -- Affinity Purification Techniques for Monoclonal Antibodies -- A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse

بدون عنوان

0

بدون عنوان

8

موضوع مستند نشده

Proteins-- Analysis, Laboratory manuals

موضوع مستند نشده

Chemistry Techniques, Analytical-- methods, Laboratory Manuals

موضوع مستند نشده

Peptides-- analysis, Laboratory Manuals

موضوع مستند نشده

Protein Conformation, Laboratory Manuals

موضوع مستند نشده

Proteins-- analysis, Laboratory Manuals

موضوع مستند نشده

Proteins-- Analysis, Laboratory manuals

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

مستند نام اشخاص تاييد نشده

Walker, John M.,1948-

تاريخ عمليات

20160712072846.0

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y