عنوان

JAK-STAT signalling :

شابک

162703241X

شابک

9781627032414

شماره

b405618

عنوان اصلي

JAK-STAT signalling :

نام عام مواد

[Book]

ساير اطلاعات عنواني

methods and protocols /

نام نخستين پديدآور

edited by Sandra E. Nicholson and Nicos A. Nicola.

محل نشرو پخش و غیره

New York :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

c2013.

نام خاص و کميت اثر

xi, 320 p. :

ساير جزييات

ill. ;

ابعاد

26 cm.

عنوان فروست

Methods in molecular biology,

عنوان فروست

Springer protocols

مشخصه جلد

967

شاپا ي ISSN فروست

1064-3745 ;

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken -- Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan -- In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy -- Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon -- Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper -- Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler -- The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks -- Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green -- Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath -- Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan -- Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg -- Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer -- Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens -- Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich -- Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier -- Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar -- Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson -- Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson -- In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw -- Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert -- Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.

بدون عنوان

0

موضوع مستند نشده

Cellular signal transduction, Laboratory manuals.

موضوع مستند نشده

Cytokines, Laboratory manuals.

موضوع مستند نشده

Protein kinases, Laboratory manuals.

موضوع مستند نشده

Janus Kinases-- metabolism, Laboratory Manuals.

موضوع مستند نشده

Signal Transduction, Laboratory Manuals.

موضوع مستند نشده

STAT Transcription Factors-- metabolism, Laboratory Manuals.

مستند نام اشخاص تاييد نشده

Nicholson, Sandra E.

مستند نام اشخاص تاييد نشده

Nicola, Nicos,1950-

تاريخ عمليات

20130211091617.0

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y