edited by Ulrich Lehmann, Medizinische Hochschule Hannover, Institute of Pathology, Hannover, Germany, Jörg Tost, Laboratory for Epigenetics and Environment, Centre National de Génotypage, CEA-Institut de Génomique, Evry, France.
وضعیت ویراست
وضعيت ويراست
Second edition.
وضعیت نشر و پخش و غیره
محل نشرو پخش و غیره
New York :
نام ناشر، پخش کننده و غيره
Humana Press,
تاریخ نشرو بخش و غیره
[2015]
فروست
عنوان فروست
Methods molecular biology,
عنوان فروست
Springer protocols
مشخصه جلد
1315
شاپا ي ISSN فروست
1064-3745 ;
يادداشت کلی
متن يادداشت
Preceded by Pyrosequencing protocols / edited by Sharon Marsh. c2007.
یادداشتهای مربوط به کتابنامه ، واژه نامه و نمایه های داخل اثر
متن يادداشت
Includes bibliographical references and index.
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
"The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology."--Back cover.