Effects of ammonia, sulfur and isoacids on in vitro ruminal fermentation of tropical forages
General Material Designation
[Thesis]
First Statement of Responsibility
S. A. Rodriguez-Medina
Subsequent Statement of Responsibility
R. M. Cook
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
Michigan State University
Date of Publication, Distribution, etc.
1992
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
108
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
M.S.
Body granting the degree
Michigan State University
Text preceding or following the note
1992
SUMMARY OR ABSTRACT
Text of Note
The effects of two levels of NH3, sulfur and isoacids on in vitro ruminal true digestibility (IVTD) of eight tropical forages was studied using a 2 factorial design. Trypticase was added to the final incubation media. This casein hydrolyzate should not have been added because it contributes NH3, isoacids and sulfur. This raised the levels of NH3 from the expected 5-10 to 17-26 mg/dl and isoacid levels about 3-fold. Sulfur levels were not affected. The results are discussed in light of trypticase addition. After a 48 h in vitro ruminal fermentation, NDF, ADF and lignin were measured to estimate IVTD. The results showed that IVTD increased with high levels of NH3 and when isoacids were added to treatments low in NH3. IVTD decreased when both NH3 and isoacids were high. High levels of sulfur tended to decrease IVTD for all forages, except elephant grass. Addition of isoacids increased total VFA concentration.