عنوان

Factors affecting protoplast isolation from citrus (lemon)

Number

TLpq304029665

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Factors affecting protoplast isolation from citrus (lemon)

General Material Designation

[Thesis]

First Statement of Responsibility

K. M. Abu-Zuhri

Subsequent Statement of Responsibility

H. B. Tepper

Name of Publisher, Distributor, etc.

State University of New York College of Environmental Science and Forestry

Date of Publication, Distribution, etc.

1992

Specific Material Designation and Extent of Item

73

Dissertation or thesis details and type of degree

M.S.

Body granting the degree

State University of New York College of Environmental Science and Forestry

Text preceding or following the note

1992

Text of Note

Thirteen factors thought to influence the release of protoplasts from leaf tissue of lemon (Citrus limon Bum.) were tested to develop a protocol to maximize protoplast yield. Maximal yields were achieved using leaves that had jut matured and were exposed to continuous light prior to and during the digestion period. A shaker speed of 100 rpm, pH of 5 and temperature of 29 1 C were the best physical parameters for leaf digestion. An osmoticum of 0.3M mannitol, 0.3M sucrose and 0.3M sorbitol was found to be better than any of the sugars individually. Different enzyme concentrations and combinations were tested and a mixture of 0.19% driselase and 0.51% pectinase was found to be most effective. Added salts and 2,4-dichlorophenoxy acetic acid had little effect on protoplast isolation.

Biological sciences

Cellular biology

Forestry

H. B. Tepper

K. M. Abu-Zuhri

Electronic name

p

[Thesis]

276903

a

Y