عنوان

Bioremediation of Spent Engine Oil in Some Selected Contaminated Soils

Number

TL51542

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Bioremediation of Spent Engine Oil in Some Selected Contaminated Soils

General Material Designation

[Thesis]

First Statement of Responsibility

Sulayman, Hikmat Omolara

Subsequent Statement of Responsibility

Ajiboye, A. E.

Name of Publisher, Distributor, etc.

Kwara State University (Nigeria)

Date of Publication, Distribution, etc.

2019

Text of Note

135 p.

Dissertation or thesis details and type of degree

M.S.

Body granting the degree

Kwara State University (Nigeria)

Text preceding or following the note

2019

Text of Note

The aim of this research is to investigate bioremediation of spent engine oil on some selected contaminated soils. Soil samples were collected from five (5) mechanic workshops along Taiwo-Oke within Ilorin metropolis. They were subjected to bioremediation using the land-farming approach. Physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Physicochemical parameters of soil samples before bioremediation included particle size (all sandy in nature); pH (5.95 ± 0.07 - 6.25 ± 0.07); Organic carbon (8.18 ± 0.31 - 17.54 ± 1.87), Organic matter (18.34 ± 2.90 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07), while values obtained after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ± 0.84, 37.25 ± 1.06 - 44.80 ± 1.13, ND - 9.40 ± 0.04 respectively. Bacterial counts before and after bioremediation of soil samples were 2.50 ± 0.71 - 67.50 ± 2.12 x 105 CFU/mL and 6.50 ± 2.12 - 164.00 ± 11.31 x 105 CFU/mL respectively. The bacterial isolates were identified as Pseudomonas sp., Acinetobacter sp., Klebsiella sp., Bacillus sp., Enterobacter sp. and Aeromonas sp. while the hydrocarbon-utilizing bacteria were identified as Thalassospira mesophila strain JCM 18969; Pseudomonas fluorescens F113; Siccibacter turicensis LMG 23730; Pseudomonas Zeshuii strain KACC 15471; Pseudomonas stutzeri strain CGMCC 1.1803 and Marinobacter hydrocarbonoclasticus strain ATCC 49840. Total petroleum hydrocarbon in the soils before and after bioremediation had retention time of 7.182 - 24.96 and 22.752 - 28.513 respectively. It is concluded that the bacteria isolated effectively bioremediated the spent engine oil contaminated soils with a reduction of hydrocarbon pollutants.

Subject Term

Bioremediation

Subject Term

Microbiology

Sulayman, Hikmat Omolara

Ajiboye, A. E.

Kwara State University (Nigeria)

Electronic name

p

[Thesis]

276903

a

Y