عنوان

Pichia protocols /

(Number (ISBN

0896034216

(Number (ISBN

1280843071

(Number (ISBN

1592595782

(Number (ISBN

6610843074

(Number (ISBN

9780896034211

(Number (ISBN

9781280843075

(Number (ISBN

9781592595785

(Number (ISBN

9786610843077

Erroneous ISBN

9781489928023

Number

b713129

Title Proper

Pichia protocols /

General Material Designation

[Book]

First Statement of Responsibility

edited by David R. Higgins and James M. Cregg.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©1998.

Specific Material Designation and Extent of Item

1 online resource (xi, 270 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

v. 103

Text of Note

Includes bibliographical references and index.

Text of Note

Introduction to Pichia pastoris / David R. Higgins, James M. Cregg -- Classical genetic manipulation / James M. Cregg, Shigang Shen, Monique Johnson, Hans R. Waterham -- Transformation / James M. Cregg, Kimberly A. Russell -- Small vectors for expression based on dominant drug resistance with direct multicopy selection / David R. Higgins, Katie Busser, John Comiskey, Peter S. Whittier, Thomas J. Purcell, James P. Hoeffler -- The generation of multicopy recombinant strains / Mike Romanos, Carol Scorer, Koti Sreekrishna, Jeff Clare -- Isolation of nucleic acids / David J. Miles, Katie Busser, Christine Stalder, David R. Higgins -- Generation of protease-deficient strains and their use in heterologous protein expression / Martin A. Gleeson, Christopher E. White, David P. Meininger, Elizabeth A. Komives -- Glycosylation profiling of heterologous proteins / José A. Cremata, Raquel Montesino, Omar Quintero, Rossana García -- High cell-density fermentation / Jayne Stratton, Vijay Chiruvolu, Michael Meagher -- Use of Pichia pastoris as a model eukaryotic system : peroxisome biogenesis / Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley, Stanley R. Terlecky, Thibaut J. Wenzel, Suresh Subramani -- Secretion of recombinant human insulin-like growth factor I (IGF-I) / Russell A. Brierley -- Secretion of scFv antibody fragments / Hermann Gram, Rita Schmitz, Rüdiger Ridder -- Expression of tetanus toxin fragment C / Jeff Clare, Koti Sreekrishna, Mike Romanos -- Expression of EGF and HIV envelope glycoprotein / Jeff Clare, Carol Scorer, Rich Buckholz, Mike Romanos -- Expression of an integral membrane protein, the 5HT5A receptor / H. Markus Weiss, Winfried Haase, Helmut Reiländer -- Localization of the myc-Tagged 5HT5A receptor by immunogold staining of ultrathin sections / Winfried Haase, H. Markus Weiss, Helmut Reiländer -- Foreign proteins expressed in P. pastoris.

0

Text of Note

The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

Title

Pichia protocols

Genetic vectors.

Pichia pastoris.

Yeast fungi-- Biotechnology.

Biotechnology.

Genetic Vectors.

Pichia.

Saccharomycetales-- genetics.

Genetic vectors.

Gist.

MEDICAL-- Microbiology.

Methylotrofe bacteriën.

Pichia pastoris.

Wetenschappelijke technieken.

Yeast fungi-- Biotechnology.

MED-- 052000

PSF.

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Cregg, James M.

Higgins, David R., (David Robert),1958-

Date of Transaction

20201208033445.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y