عنوان

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides.

Number

LA8vv700bs

Title Proper

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides.

General Material Designation

[Article]

First Statement of Responsibility

Coradetti, Samuel TPinel, DominicGeiselman, Gina MIto, MasakazuMondo, Stephen JReilly, Morgann CCheng, Ya-FangBauer, StefanGrigoriev, Igor VGladden, John Met al.

Text of Note

The basidiomycete yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) accumulates high concentrations of lipids and carotenoids from diverse carbon sources. It has great potential as a model for the cellular biology of lipid droplets and for sustainable chemical production. We developed a method for high-throughput genetics (RB-TDNAseq), using sequence-barcoded Agrobacterium tumefaciens T-DNA insertions. We identified 1,337 putative essential genes with low T-DNA insertion rates. We functionally profiled genes required for fatty acid catabolism and lipid accumulation, validating results with 35 targeted deletion strains. We identified a high-confidence set of 150 genes affecting lipid accumulation, including genes with predicted function in signaling cascades, gene expression, protein modification and vesicular trafficking, autophagy, amino acid synthesis and tRNA modification, and genes of unknown function. These results greatly advance our understanding of lipid metabolism in this oleaginous species and demonstrate a general approach for barcoded mutagenesis that should enable functional genomics in diverse fungi.

Date of Publication

2018

Title

UC Berkeley

Electronic name

[Article]

279260

a

Y