عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Number
LA2h34n1jx
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Combined aptamer and transcriptome sequencing of single cells.
General Material Designation
[Article]
First Statement of Responsibility
Delley, Cyrille LLiu, LeqianSarhan, Maen FAbate, Adam R
SUMMARY OR ABSTRACT
Text of Note
The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.
SET
Date of Publication
2018
Title
UCSF
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Article]
277652
a
Y
