عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Number
TL40k6t4s9
LANGUAGE OF THE ITEM
.Language of Text, Soundtrack etc
انگلیسی
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Cell type-specific analysis of gene expression in an in vitro model of neurogenesis
General Material Designation
[Thesis]
First Statement of Responsibility
Tang, Sophia M.
Subsequent Statement of Responsibility
Ardell, David H.
.PUBLICATION, DISTRIBUTION, ETC
Date of Publication, Distribution, etc.
2010
DISSERTATION (THESIS) NOTE
Body granting the degree
Ardell, David H.
Text preceding or following the note
2010
SUMMARY OR ABSTRACT
Text of Note
The project attempts to answer the following question: What are the neural stem cell (NSC) transcription factors (TFs) that determine neurogenesis and those that determine astrogenesis? NSCs co-exist with other neural cell types during differentiation and communicate with other cells in the NSC niches. Furthermore, expressions of the NSC TFs are time dependent. These conditions make it very challenging to analyze TFs of NSCs. The technique I will use to address the challenge is called TU-tagging, which utilizes the UPRT enzyme from Toxoplasma gondii that is not found in mammalian cells to incorporate a thio-tag into the mRNA only in a specific cell type. A long-term aim is to apply TU-tagging to extract cell type-specific mRNAs in the in vitro model of neural differentiation. In my work, preliminary experiments have been performed and reagents have been generated to help meet this goal. Ubiquitous and cell type-specific UPRT expression vectors for TU-tagging have been made and were used to validate the method using a P19 cell line that is a traditional model of neural differentiation.
PERSONAL NAME - PRIMARY RESPONSIBILITY
Cornell, Devin J.
PERSONAL NAME - SECONDARY RESPONSIBILITY
Tang, Sophia M.
CORPORATE BODY NAME - SECONDARY RESPONSIBILITY
UC Merced
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب p
[Thesis]
276903
a
Y
