The Three-dimensional Structure of Proteins -- Chemical Catalysis -- The Basic Equations of Enzyme Kinetics -- Measurement and Magnitude of Enzymatic Rate Constants -- The pH Dependence of Enzyme Catalysis -- Practical methods for Kinetics and equilibria -- Detection of Intermediates in Enzymatic Reactions -- Stereochemistry of Enzymatic Reactions -- Active-site-directed and Enzyme-activated Irreversible Inhibitors : Affinity Labels and Suicide Inhibitors -- Conformational Change, Allosteric Regulation, Motors, and Work -- Forces Between Molecules and Binding Energies -- Enzyme-substrate Complementarity and the Use of Binding Energy in Catalysis -- Specificity and Editing Mechanisms -- Recombinant DNA Technology -- Protein Engineering -- Case studies of enzyme structure and mechanism -- Protein Stability -- Kinetics of Protein Folding -- Folding Pathways and Energy Landscapes
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SUMMARY OR ABSTRACT
Text of Note
Includes four new chapters which focus on the repercussions of protein engineering for protein folding and catalysis, this new edition is a more general guide to mechanism in protein science