عنوان

Dictyostelium discoideum protocols

(Number (ISBN

1588296237 (alk. paper)

(Number (ISBN

1597451444 (e-ISBN)

(Number (ISBN

9781588296238

(Number (ISBN

9781597451444 (e-ISBN)

Number

dltt

Title Proper

Dictyostelium discoideum protocols

General Material Designation

[Book]

First Statement of Responsibility

edited by Ludwig Eichinger, Francisco Rivero.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

c2006.

Specific Material Designation and Extent of Item

xv, 564 p. :

Other Physical Details

ill. ;

Dimensions

24 cm.

Series Title

Methods in molecular biology,

Volume Designation

346

ISSN of Series

1064-3745 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Preparation of intact, highly purified phagosomes from dictyostelium / Daniel Gotthardt ... [et al.] -- Assaying cell-cell adhesion / Salvatore Bozzaro -- Periodic activation of ERK2 and partial involvement of G protein in ERK2 activation by cAMP in dictyostelium cells / Mineko Maeda -- An improved method for dictyostelium centrosome isolation / Irene Schulz ... [et al.] -- Epigenetics in dictyostelium / Markus Kaller, wolfgang Nellen, and Jonathan R. Chubb -- Dictyostelium discoideum as a model to study host-pathogen interactions / Can Ünal and Michael Steinert -- Pharmacogenetics: defining the genetic basis of drug action and inositol trisphosphate analysis / Kathryn E. Adley, Melanie Keim, and Robin S.B. Williams -- How to assess and study cell death in dictyostelium discoideum / Artemis Kosta ... [et al.].

Text of Note

Pt. I. The organism, community resources, and genome-wide studies. The secret lives of dictyostelium / Richard H. Kessin -- the genome of dictyostelium discoideum / Adam Kuspa and William F. Loomis -- The cDNA sequencing project / Hideko Urushihara, Takahiro Morio, and Yoshimasa Tanaka -- dictyBase and the dicty stock center / Petra Fey ... [et al.] -- Analysis of gene expression using cDNA microarrays / Marcel Kaul and Ludwig Eichinger -- Proteomic analysis of dictyostelium discoideum / Udo Roth, Stefan Müller, and Franz-Georg Hanisch -- Pt. II. Basic methods and molecular genetic techniques. Cultivation, spore production, and mating / Hideko Urushihara -- Parasexual genetics using axenic cells / Jason King and Robert Insall -- Slug phototaxis, thermotaxis, and spontaneous turning behavior / Paul R. Fisher and Sarah J. Annesley -- Purification techniques of subcellular compartments for analytical and preparative purposes / Laurence Aubry and Gérard Klein -- Generation of multiple knockout mutants using the cre-loxP system / Alan r. Kimmel and Jan Faix --

Text of Note

Restriction enzyme-mediated integration (REM1) mutagenesis / Adam Kuspa -- RNA interference and antisense-mediated gene silencing in dictyostelium / Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen -- Pt. III. Imaging and localization methods. Application of fluorescent protein tags as reporters in live-cell imaging studies / Annette Müller-Taubenberger -- Investigating gene expression: in situ hybridization and reporter genes / Ricardo Escalante and Leandro Sastre -- Application of 2D and 3D DIAS to motion analysis of live cells in transmission and confocal microscopy imaging / Deborah Wessels, Spencer Kuhl, and David R. Soll -- Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells / Xuehua Xu, Joseph A. Brzostowski, and Tian Jin -- Visualizing signaling and cell movement during the multicellular stages of dictyostelium development / Dirk Dormann and Cornelis J. Weijer --

Text of Note

Under-agarose chemotaxis of dictyostelium discoideum / David Woznica and David A. Knecht -- Optimized fixation and immunofluorescence staining methods for dictyostelium cells / Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati -- Cryofixation methods for ultrastructural studies of dictyostelium discoideum / Mark J. Grimson and Richard L. Blanton -- Pt. IV. Dictyostelium as model organism. Analysis of signal transduction: formation of cAMP, cGMP, and Ins(1,4,5)P, in vivo and in vitro / Peter J.M. Van Haastert -- Assaying chemotaxis of dictyostelium cells / Michelle C. Mendoza and Richard A. Firtel -- Characterization of cross-linked actin filament gels and bundles using birefringence and polarized light scattering / Ruth Furukawa and Marcus Fechheimer -- Quantitative and microscopic methods for studying the endocytic pathway / Francisco Rivero and Markus Maniak --

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Text of Note

Mode of access: World Wide Web.

Title

Dictyostelium discoideum protocols.

Title

OhioLINK electronic book center

Title

SpringerLink

Dictyostelium discoideum, Laboratory manuals.

Eichinger, Ludwig.

Rivero-Crespo, Francisco.

Date of Transaction

20080604110559.0

Electronic name

[Book]

Y