عنوان

PIWI-interacting RNAs

(Number (ISBN

9781627036931 )alk. paper(

Title Proper

PIWI-interacting RNAs

First Statement of Responsibility

edited by Mikiko C. Siomi, Graduate School of Science, The University of Tokyo, Tokyo, Japan

Name of Publisher, Distributor, etc.

Springer Science]Business Media

Date of Publication, Distribution, etc.

2014

Specific Material Designation and Extent of Item

xi, 249 pages : illustrations )some color(

Series Title

Methods in molecular biology,1064-3745 ;1093

Series Title

Springer protocols

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1093.1064-3745

Series Title

Series: Springer protocols )Series(1949-2448

Text of Note

Chromatin Immunoprecipitation Assay of Piwi in Drosophila /Hang Yin and Haifan Lin --Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-Mediated RNAi /Yuzo Niki, Takuya Sato, Takafumi Yamaguchi, Ayaka Saisho, Hiroshi Uetake, and Hidenori Watanabe --RNAi and Overexpression of Genes in Ovarian Somatic Cells /Kuniaki Saito --Making piRNAs In Vitro /Shinpei Kawaoka, Susumu Katsuma, and Yukihide Tomari --A Framework for piRNA Cluster Manipulation /Ivan Olovnikov, Adrien Le Thomas, and Alexei A. Aravin --Biochemical and Mass Spectrometric Analysis of 3س‌-End Methylation of piRNAs /Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, and Tsutomu Suzuki --HITS-CLIP )CLIP-Seq( for Mouse Piwi Proteins /Anastassios Vourekas and Zissimos Mourelatos --DNA Methylation in Mouse Testes /Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, and Toru Nakano --Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes /Michael Reuter and Ramesh S. Pillai --Small RNA Library Construction from Minute Biological Samples /Jessica A. Matts, Yuliya Sytnikova, Gung-wei Chirn, Gabor L. Igloi, and Nelson C. Lau --Analysis of sDMA Modifications of PIWI Proteins /Shozo Honda, Yoriko Kirino, and Yohei Kirino --Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries /Sergey Shpiz and Alla Kalmykova --Combined RNA/DNA Fluorescence In Situ Hybridization on Whole-Mount Drosophila Ovaries /Shpiz, Sergey Lavrov, and Alla Kalmykova --Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries /Thomas Grentzinger and Saeverine Chambeyron --Isolation of Zebrafish Gonads for RNA Isolation /Hsin-Yi Huang and Renae F. Ketting --Small RNA Library Construction for High-Throughput Sequencing /Jon McGinn and Benjamin Czech --Analysis of Piwi-Loaded Small RNAs in Terahymena /Tomoko Noto, Henriette M. Kurth, and Kazufumi Mochizuki --Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs /Hailong Wang, Haidong Huang, and Dahua Chen --Isolation and Bioinformatic Analyses of Small RNAs Interacting with Germ Cell-Specific Argonaute in Rice /Reina Komiya and Ken-Ichi Nonomura.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Small interfering RNA^aLaboratory manuals

Entry Element

Piwi genes^aLaboratory manuals

Entry Element

RNA, Small Interfering^aLaboratory Manuals

Entry Element

Models, Animal^aLaboratory Manuals

Entry Element

Laboratory Manuals.^amesh

Entry Element

Piwi genes.^afast--)OCoLC(fst01200830

Entry Element

Small interfering RNA.^afast--)OCoLC(fst01121445

Entry Element

Handbooks, manuals, etc.^afast--)OCoLC(fst01423877

Number

Class number

Classification Record Number

Book number

Siomi, Mikiko C.

English Book