عنوان

Molecular basis for the cachexia disease syndrome of citrus

شماره

TLpq304353782

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Molecular basis for the cachexia disease syndrome of citrus

نام عام مواد

[Thesis]

نام نخستين پديدآور

K. Reanwarakorn

نام ساير پديدآوران

J. S. Semancik

نام ناشر، پخش کننده و غيره

University of California, Riverside

تاریخ نشرو بخش و غیره

1997

نام خاص و کميت اثر

161

جزئيات پايان نامه و نوع درجه آن

Ph.D.

کسي که مدرک را اعطا کرده

University of California, Riverside

امتياز متن

1997

متن يادداشت

Citrus viroid group II with 295-302 nts, consists of cachexia-inducing variants, CVd-IIb (Citrus cachexia viroid, CCaVd), CVd-IIc, Ca-903, Ca-909 and a single non-cachexia inducing variant, CVd-IIa. Parson's special mandarin (PSM) expressed gumming and stem pitting when inoculated only with cachexia-inducing variants. CVd-IIa infection resulted in strong symptoms of short internodes and rugous leave on luffa (Luffa aegyptiaca) while cachexia variants induced mild symptoms. Cachexia-inducing variants, CVd-IIb and CVd-IIc produced classical cachexia type symptoms of gumming and stem pitting on PSM, Orlando tangelo (OT) and Citrus macrophylla. However, Palestine sweet lime (PSL), the indicator of xyloporosis, displayed a simple fine pitting reaction with infection by both CVd-IIb and CVd-IIc. The nucleotide sequence of CVd-IIc although differing from CVd-IIb was identical with a variant common to xyloporosis sources from Israel. Thus, these studies suggest that cachexia and xyloporosis should be concluded as the same disease. The group II citrus viroids are characterized by a very conservative genome since only a few base changes were detected after passaging through the four different families of plants known to be hosts of hop stunt viroid (HSVd). Five variants from naturally-infected grapevine have been found and determined to contain in 295-297 bases. One HSVd-gVl, was negative for cachexia symptoms on PSM, but induced mild symptoms on PSM, but induced mild symptoms on Mapo tangelo what might represent a more sensitive indexing host than PSM. Chimeric viroid cDNA clones carrying the C-V-T2 domain region from IIa-clone induced severe symptoms on luffa, while clones with a similar genome region from IIb-clone induced very strong cachexia symptoms on PSM. In site-directed mutated cDNA clones, the six mutagenic loci introduced into the V-domain of the IIa-clone were successful in the induction of pathogenicity on PSM. From this, the C-V-T2 domains of CVd-IIa and CVd-IIb represent an important locus for regulating pathogenicity on both luffa and PSM. Probe CX2 designated as a complementary to CVd-IIb at position 182-208 was demonstrated to function as a cachexia-specific probe. The sequence as contain in the probe CX2 was tested as a cachexia specific primer for RT-PCR as both a cDNA and hDNA. When the CX2 sequence was used as a hDNA primer, a cachexia-specific product resulted under specific conditions of 1.0 mM MgCl2 at 55C.

موضوع مستند نشده

Biological sciences

موضوع مستند نشده

Botany

موضوع مستند نشده

Plant pathology

مستند نام اشخاص تاييد نشده

J. S. Semancik

مستند نام اشخاص تاييد نشده

K. Reanwarakorn

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y