عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL56636
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Characterization and Isolation of Segmented Filamentous Bacteria from Commercial Turkey
نام عام مواد
[Thesis]
نام نخستين پديدآور
Hedblom, Grant Andrew
نام ساير پديدآوران
Baumler, David J.
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Minnesota
تاریخ نشرو بخش و غیره
2020
يادداشت کلی
متن يادداشت
224 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Minnesota
امتياز متن
2020
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
This work aimed to characterize and isolate segmented filamentous bacteria from turkey hosts in order to determine the role these organisms play in improving the health of commercial turkeys. Segmented filamentous bacteria (SFB) have been associated with healthy growth outcomes of turkey flocks and are assumed to play an immunostimulatory role in the development of the innate and adaptive immune systems of the host and have potential as novel direct-fed microbials as the demand for alternatives to antibiotic use rises. The approach involved several methods for the in vitro and in silico analysis of turkey-associated SFB, including whole genome sequencing, predictive genomics and metabolomics assays, extraction and enrichment of SFB from ileal homogenates, development of a genetic detection technique, and comparative growth studies in various media formulations. Additionally, attempts to isolate SFB-turkey from other members of the extracted intestinal co-cultures were attempted using dilution-to-extinction, growth in "spent media", separation via filtration and targeted use of species-specific bacteriophage lysates. Turkey-associated SFB are very distinct among the SFB, possessing several distinguishing metabolic characteristics including an increased capacity to degrade amino acids, metabolize a broader array of mono/disaccharide substrates, break down complex glycans, hydrolyze and recycle primary bile acids, and synthesize biotin. In examining the ability of SFB-turkey to grow and reproduce in vitro, it was initially determined that turkey- associated SFB were able to grow to high proportions independently from intestinal cell lines in liquid cultures grown under microaerobic conditions after 48 hours of incubation. Following several failed attempts to separate and purify SFB-turkey from other microbial members contained within the obtained co-cultures it was determined that cultures presumed to contain SFB-turkey were found to consist of high proportions of Bacillus marisflavi, an organism observed to display filamentous growth without the formation of transverse septa. The presented work underscores the highly auxotrophic nature of SFB and overall dependence on the host intestinal environment for consistent growth and development.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Animal sciences
اصطلاح موضوعی
Biochemistry
اصطلاح موضوعی
Developmental biology
اصطلاح موضوعی
Food science
اصطلاح موضوعی
Genetics
اصطلاح موضوعی
Immunology
اصطلاح موضوعی
Microbiology
اصطلاح موضوعی
Organic chemistry
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Hedblom, Grant Andrew
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Baumler, David J.
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
University of Minnesota
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
