عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL51197
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Analysis of Rift Valley Fever Virus Host-Pathogen Dynamics
نام عام مواد
[Thesis]
نام نخستين پديدآور
Havranek, Katherine Elizabeth
نام ساير پديدآوران
Lodmell, J. Stephen
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Montana
تاریخ نشرو بخش و غیره
2019
يادداشت کلی
متن يادداشت
129 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Montana
امتياز متن
2019
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Rift Valley fever virus (RVFV) is an emerging pathogen that causes severe disease in humans and domestic livestock. There is no licensed treatment or vaccine to combat RVFV infection available for human use. RVFV is endemic to sub-Saharan Africa, but in recent years outbreaks have extended throughout Africa and onto the Arabian Peninsula. The virus has been shown to be transmitted by a diversity of mosquito species, including those that are present in the Western Hemisphere. This demonstrated potential for spread and the potential health and economic impacts of outbreaks compels a greater understanding of RVFV molecular virology and development of therapeutic options. The virally encoded nucleocapsid protein (N) has several known functions at critical stages of the viral replication cycle. Each of these important roles involves the ability of N to bind RNA. Thus the N-RNA interface is an intriguing molecular target for antiviral therapeutic intervention. The work described here focuses on characterization of N-RNA interactions, inhibition of these interactions, and the possible role of N or other viral proteins in diminishing the cellular antiviral response. We carried out a small molecule screen and identified a compound that diminished the ability of N to interact with RNA. We also executed a crosslinking and immunoprecipitation with high-throughput sequencing experiment to investigate N-RNA interactions in infected cells. This study allowed us to propose specific sequence motifs as putative N recognition sites. This work further indicated that N protein is not only a critical component of the viral replication machinery, but its extensive interaction with host RNAs could be a previously unappreciated viral mechanism for disrupting host RNA metabolism and processing. The unexpected affinity of N for host RNAs led us to perform transcriptome profiling in RVFV infected cells, which highlighted pervasive changes in gene expression and alternative splicing that occur as a result of infection. We examined alternative splicing during RVFV infection on both a global scale and in detail with a particular host gene, RIOK3, which we discovered to be an important antiviral protein whose expression and processing are altered upon RVFV infection. This work adds to a new but growing body of knowledge that recognizes splicing as an important yet understudied mechanism that influences host-pathogen interactions.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Biology
اصطلاح موضوعی
Genetics
اصطلاح موضوعی
Microbiology
اصطلاح موضوعی
Molecular biology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Havranek, Katherine Elizabeth
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Lodmell, J. Stephen
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
University of Montana
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
