عنوان

Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications

شماره

LA3b0743s3

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications

نام عام مواد

[Article]

نام نخستين پديدآور

Haduong, Julie; Nayak, Rahul; Suh, Yumi; Viswanadham, Vinay

متن يادداشت

A new approach to whole-scale organ engineering is the seeding of stem cells upon a decellularized scaffold of a desired organ. Current efforts seek to use this method for whole-scale organ engineering. Here, we report a novel decellularizationrecellularization approach to engineering a human colonic graft with human enterocytes and xenogeneic scaffold. Porcine colon tissue was decellularized with protein-solubilizing agents and recellularized by incubation with Caco-2. Immunohistochemistry, DNA staining, and SEM were used to study the effects upon the scaffold. Exposure to 2%SDS-0.5%Triton solution for four hours resulted in the best decellularization and preservation of microstructure, while tissues treated with 2%SDS-0.5%Triton and 0.05%Trypsin/EDTA supported the best recellularization. Our method provides a potential strategy for streamlined regeneration of a viable colonic lumen from xenogeneic and allogeneic sources while maintaining colon microstructure and potential for recellularization.

تاريخ نشر

2014

عنوان

Berkeley Scientific Journal

شماره جلد

19/1

عنصر شناسه اي

Haduong, Julie; Nayak, Rahul; Suh, Yumi; Viswanadham, Vinay

نام الکترونيکي

نوع ماده

[Article]

کد کاربرگه

275578

سطح دسترسي

a

تكميل شده

Y