عنوان

DNA repair protocols

شابک

9781617799976 )hbk. : alk. paper(

عنوان اصلي

DNA repair protocols

نام نخستين پديدآور

edited by Lotte Bjergbaek

وضعيت ويراست

3rd ed.

محل نشرو پخش و غیره

New York

نام ناشر، پخش کننده و غيره

Humana Press/Springer,

تاریخ نشرو بخش و غیره

c2012

نام خاص و کميت اثر

xvii, 631 p. : ill.

عنوان فروست

Methods in molecular biology,1064-3745 ;920

عنوان فروست

Springer protocols

عنوان فروست

Series: Methods in molecular biology )Clifton, N.J.( ;920.

عنوان فروست

Series: Springer protocols.

متن يادداشت

DNA repair : front and center and not going away! /Vilhelm A. Bohr --Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome /Dashnamoorthy Ravi and Alexander James Roy Bishop --Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening /Lone Vedel Sch▓ler, Tine H▓rning M▓ller, Steffen N▓rgaard, Lotte Vestergaard, and Anders Olesn --Establishment of the DNA repair-defective mutants in DT40 cells /Masamichi Ishiai, Emi Uchida, and Minoru Takata --Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair /Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu --Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair /Geunter Speit and Andreas Rothfuss --Detection of DNA damage by comet fluorescence in situ hybridization /Wiebke Schleormann and Michael Glei --Quantitative DNA damage and repair measurement with the yeast comet assay /Rui Oliveira and Bjeorn Johansson --Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR /Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten --In vitro DNA mismatch repair in human cells /Liya Gu, Charles M. Ensor, and Guo-Min Li --Eukaryotic DNA mismatch repair in vitro /Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang --Immuno-slot blot assay for detection of UVR-mediated DNA damage /Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke --Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay /Thomas R. Berton and David L. Mitchell --Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR /Ahmad Besaratinia and Gerd P. Pfeifer --Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies /Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schearer --Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts /Puck Knipscheer, Markus Reaschle, Orlando Schearer, and Johannes C. Walter --In vitro base excision repair using mammalian cell extracts /Jason L. Parsons and Grigory L. Dianov --Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro /Dolores Caordoba-Caonero, Teresa Roldaan-Arjona, and Rafael R. Ariza --In vitro monitoring of base excision repair in Saccharomyces cerevisiae /Simone Moertl and Fred Ahne --Mitochondrial base excision repair assays /Ricardo Gredilla and Tinna Stevnsner --O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks /April M. Luke and Jun Nakamura --DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis /Helen E. Bryant --Quantitative amplification of single-stranded DNA /Eva-Maria Holstein and David Lydall --Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast /James M. Dewar and David Lydall --Monitoring DNA recombination initiated by HO endonuclease /Neal Sugawara and James E. Haber --Monitoring I-SceI-induced double-strand break repair in DT40 Cells /David du Plessis and Julian E. Sale --I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks /Amanda Gunn and Jeremy M. Stark --Studying repair of a single protein-bound nick in vivo using the Flp-nick system /Ida Nielsen, Anni H. Andersen, and Lotte Bjergb╡k --Measurement of diversification in the immunoglobulin light chain gene of DT40 cells /Julian E. Sale --Live cell microscopy of DNA damage response in Saccharomyces cerevisiae /Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby --Use of gene targeting to study recombination in mammalian cell DNA repair mutants /Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn --In vitro rejoining of double strand breaks in genomic DNA /George Iliakis, Emil Mladenov, and Nge Cheong --An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides /Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters --Assays of bypass replication of genotoxic lesions in cell-free extracts /Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone --Quantitative measurement of translesion DNA synthesis in mammalian cells /Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh --Detection of PCNA modifications in Saccharomyces cerevisiae /Adelina A. Davies and Helle D. Ulrich --In vitro PCNA modification assays /Joanne L. Parker and Helle D. Ulrich --Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication /George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang --CHK1 kinase activity assay /Hong Yan Wang and Ya Wang --Histone H2AX phosphorylation : a marker for DNA damage /Arishya Sharma, Kamini Singh, and Alexandru Almasan.

متن يادداشت

Includes bibliographical references and index.

عنصر شناسه ای

DNA repair^aLaboratory manuals

عنصر شناسه ای

DNA Repair^aLaboratory Manuals

شماره

شماره رده

شماره رکورد رده بندي

نشانه اثر

شماره رکورد غير از شماره رده بندي

عنصر شناسه اي

Bjergb╡k, Lotte

نوع ماده

English Book