Fast masking of repeated primer binding sites in eukaryotic genomes / Reidar Andreson, Lauris Kaplinksi, and Maido Remm -- Primer design for PCR reactions in forensic biology / Kelly M. Elkins -- Design of primers and probes for quantitative real-time PCR methods / Alicia Rodriguez, Mar Rodriguez, Juan J. Cordoba, and Maria J. Andrade -- Large-scale neucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus / Alexander Nagy, Tomas Jirinec, Lenka Cernikova, Helena Jirincova, and Martina Havlickova -- Low-concentration initiator primers improve the amplification of gene targets with high sequence variability / Kenneth E. Pierce and Lawrence J. Wangh -- Multiplex PCR primer design for simultaneousu detection of multiple pathogens / Wenchao Yan -- Degenerate primer design for highly variable genomes / Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell -- Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection / Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu -- MultiPLX: automatic grouping and evaluation of PCR primers / Lauris Kaplinski and Maido Remm -- In silico PCR primer designing and validation / Anil Kumar and Nikita Chrodia -- Primer design using Primer Express for SYBR green-based quantitative PCR / Amarjeet Singh and Girdhar K. Pandey -- Designing primers for SNaPshot technique / Greciane Gaburro Paneto and Francisco de Paula Careta -- Rapid and simple method of qPCR primer design / Brenda Thornton and Chhandak Basu -- PRIMEGENSw3: a web-based tool for high-throughput primer and probe design / Garima Kushwaha, Gyan Prakash Srivastava, and Dong Xu -- Selecting specific PCR primers with MFEprimer / Wubin Qu and Chenggang Zhang