بررسی ارتباط پلی مورفیسم ژن CXCR۱ با بیماریهای رحمی پس از زایمان در گاوهای شیری
/لقمان فیضی
: دامپزشکی
، ۱۳۹۴
، راشدی
چاپی
کارشناسی ارشد
دامپزشکی
۱۳۹۴/۱۱/۱۹
تبریز
The management of cows during the transition period is important to protect them from various postpartum uterine diseases. The transition period is defined as the 3 weeks before and the 3 weeks after parturition, and represents a time of physiological stress for the dairy cow.Postpartum bacterial contamination of the uterine environment might reach 100 of cows in intensively managed dairy herds. The improper balance between uterine infection and the intrauterine antimicrobial selfdefense mechanisms often lead to the main postpartum reproductive diseases such as puerperal metritis, clinical endometritis (CE), pyometra, and subclinical endometritis. The extent of uterine disease within a week of parturition (metritis) was up to 40 Retained placenta (ROP) has been found to be the single largest postpartum complication in the bovine species and the incidence varied from 3.6 to 29 in dairy cows .The prevalence of clinically relevant endometritis between 15 and 60 days postpartum was 25.9 , but the risk of infertility and/ or sterility increased if it occurred after 14 days of parturition. However, most cows remained healthy during the postpartum period even though there is a 100 chance of bacterial contamination around calving days. Innate immunity of the cows might have taken the predominant role to protect them from the development of postpartum reproductive disease (PRD). The ability to select disease resistant cattle based on genetic information has been much discussed in recent years. Genes associated with neutrophil function are potential genetic markers for mastitis as neutrophil migration from blood to the site of infection is essential for resolution of postpartum reproductive diseases.The bovine CXCR1 gene is a potential candidate gene for mastitis resistance as it encodes the CXCR1 chemokine receptor, which is involved in neutrophil activation and chemotaxis during infection To date, there have been no studies examining the association of bovine SNPs with disease incidence. A 311 bp segment located within the coding region of exon 3 of the CXCR1 gene was amplified and sequenced.Five SNPs were expressed in both breeds of cattle.Therefore, the bovine CXCR1 gene is an excellent candidate genetic marker for mastitis resistance and/or susceptibility in dairy cattle.To specifically amplify the 311-bp fragment of bovine CXCR1 containing the SNP of interest, the following cycling parameters were used .Products of the PCR were digestedwith Hinf 1 .The digestion resulted in products of 161 and 150 bp for the G allele and 150, 111, and 50 bp for the C allele, allowing genotyping of each animal for the SNP of interest