9783527319411 (cased)
IR
E-6635
انگلیسی
IR
Proteomics in practice
[Book]
:a guide to successful experimental design
/ edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Heopker
2nd ed.
Weinheim
: Wiley-VCH ;Chichester :John Wiley [distributor]
, 2008.
xx, 482 p. , ill. (some col.) , 25 cm.
Previous ed.: 2002.
Print
Includes bibliographical references and index.
Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- Pt. III. Trouble shooting -- Glossary of terms.
Proteins- Analysis
Molecular biology
Proteomics
Proteins, Synthesis
Molecular Biology
QP551
.
P75673
2008
Westermeier, Reiner
Naven, Tom
Heopker, Hans-Rudolf
ایران
old catalog
p
BL
1
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