عنوان

3D Microscopy of Nanoparticles in Tumours

TLpq2322140835

انگلیسی

3D Microscopy of Nanoparticles in Tumours

[Thesis]

Syed, Abdullah Muhammad

Chan, Warren CW

University of Toronto (Canada)

2019

140

Ph.D.

University of Toronto (Canada)

2019

The structure of solid tumours is a major factor contributing to poor treatment outcomes because it can prevent therapeutics from reaching cancer cells. Nanoparticles are able to increase the accumulation of therapeutics in the tumour as a whole but are not able to deliver therapeutics to cancer cells because of structural barriers within the tumour. However, researchers have been unable to visualize these structures within the tumour and how it impacts nanoparticle distribution because tumours are opaque. Here, I present a series of techniques that allow the structure of the tumour and the distribution of nanoparticles within to be visualized and quantified. I developed methods to 1) render tumour tissues transparent at the scale of 100s of tissues per month needed for biological experiments, 2) quantify and improve the retention of nanoparticles in the tumours after process, 3) label and visualize nanoparticles within the tumours. These methods can now be used to gain a deeper understanding of how the structure of the tumour is organized, how it impacts nanoparticle distribution and how it can be altered to achieve optimal cancer therapy.

Biomedical engineering

Nanotechnology

Chan, Warren CW

Syed, Abdullah Muhammad

p

[Thesis]

276903

a

Y