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TL85s7624x
انگلیسی
Rats with confirmed temporal lobe epilepsy have impaired pattern separation behavior on 8 arm radial maze
[Thesis]
Ramezani, Ario Dustin
Leutgeb, Jill
UC San Diego
2017
UC San Diego
2017
Episodic memories are a unique subtype of memory that encode the when, the where, and the what. The dentate gyrus within the hippocampus is vital for mediating pattern separation: the brain's distinct ability to distinguish very similar memories from one another. Epilepsy, a disease characterized by hyper-excitation of neuronal circuits, is correlated with neuronal reorganization of the dentate gyrus. Previous studies from our lab have demonstrated that rats induced with chronic temporal lobe epilepsy through a low-dose kainate model have impaired behavioral performance on an 8 arm radial maze that tests for pattern separation. In this project, we asked whether these behavioral deficits were a precursor to, or a result of, chronic epileptogenesis. We introduced a 24-hour video monitoring system, which allowed us to review recorded video of induced animals in a vivarium to observe and score seizures. This data was then compared to the data collected from behavioral trials and we found that in the adjacent condition of the 8 arm radial maze (pattern separation condition), those with confirmed epilepsy had a statistically significant increase in trials necessary to reach criterion when compared to the control group. Their performance was comparable to a dentate gyrus lesion group, which controlled for inhibited pattern separation function. In the non-adjacent 8 arm radial maze condition, there was no significant difference in performance between groups since this condition is not dentate gyrus-dependent. Holistically, we conclude that chronic epileptogenesis results in impaired pattern separation behavior. Histological analysis of Timm stained tissue further supports that mossy fiber sprouting is positively correlated with impaired pattern separation behavior.
Ramezani, Ario Dustin
Leutgeb, Jill
UC San Diego
مطالعه متن کتاب p
[Thesis]
276903
a
Y
