عنوان
پدید آورنده
موضوع
رده
QH442
QH442
کتابخانه
محل استقرار
1351021206
1351021214
1351021222
9781351021203
9781351021210
9781351021227
081537075X
9780815370758
Advances in Molecular Techniques /
[Book]
Rakesh S. Sengar, Amit Kumar, Reshu Chaudhary, Ashu Singh.
First edition.
Boca Raton, FL :
CRC Press,
2018.
1 online resource :
text file, PDF
Includes bibliographical references and index.
Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation.
Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions.
Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals.
MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required.
Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required.
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"Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists. This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering."--Provided by publisher.
Ingram Content Group
9781351021203
9781351021203
Biotechnology.
Genetics.
Molecular biology.
Computational Biology-- methods.
Genetic Techniques.
Biotechnology.
Genetics.
Molecular biology.
SCIENCE-- Life Sciences-- Biochemistry.
MED009000
SCI-- 007000
SCI029000
SCI049000
SCBS0755
SCBS0770
SCBS09
WB042
WB057
WB061
WB071
WB075
QH442
2018
G-016
QU
550
Sengar, Rakesh S.
Chaudhary, Reshu
Kumar, Amit
Singh, Ashu
20200822134800.0
pn
مطالعه متن کتاب [Book]
Y
