عنوان

Practical Plant Virology :

3642720307

9783642720307

b573055

Practical Plant Virology :

[Book]

Protocols and Exercises

by Jeanne Dijkstra, Cees P. Jager.

Berlin, Heidelberg

Springer Berlin Heidelberg

1998

(xvi, 459 pages 77 illustrations)

Springer lab manual.

I Virus Inoculation --; Protocol 1 Mechanical Inoculation of Plants --; Protocol 2 Inoculation of Protoplasts Isolated from Leaves --; Protocol 3 Differential Temperature Treatment of Infected Plants --; Exercise 1 Infectivity of Tobacco Mosaic Tobamovirus --; II Symptomatology --; Protocol 4 Description of External Symptoms --; Protocol 5 Determination of Internal Symptoms --; Exercise 2 Systemic Acquired Resistance (SAR) --; Exercise 3 Demonstration of Phloem Necrosis in Plants Affected by Potato Leafroll --; Exercise 4 Inclusions in Hair Cells of Leaves of Tobacco Infected with Tobacco Mosaic Tobamovirus --; III Infectivity Assays --; Protocol 6 Infectivity Assay on Local-Lesion Hosts --; Protocol 7 Infectivity Assay on Hosts Producing Starch Lesions --; Protocol 8 Infectivity Assay on Hosts Producing Systemic Symptoms Only --; Exercise 5 Effect of Temperature on the Susceptibility of Plants --; Exercise 6 Effect of Abrasives on the Infection --; Exercise 7 Effect of Inhibitors on the Infection --; Exercise 8 Effect of Light on the Susceptibility of Plants --; Exercise 9 Effect of Time of the Day on the Susceptibility of Plants --; IV Infectivity of Virus in Crude Sap --; Protocol 9 Determination of the Dilution End-Point --; Protocol 10 Determination of the Thermal Inactivation Point --; Protocol 11 Determination of the Longevity In Vitro --; Exercise 10 Determination of the Dilution End-Point --; Exercise 11 Determination of the Thermal Inactivation Point --; Exercise 12 Determination of the Longevity In Vitro --; V Transmission of Viruses --; Protocol 12 Virus Transmission by Grafting --; Protocol 13 Virus Transmission Through Soil --; Protocol 14 Virus Transmission by Nematodes --; Protocol 15 Virus Transmission by Fungi --; Protocol 16 Virus Transmission by Aphids --; Protocol 17 Transmission by Leafhoppers (Cicadellidae) --; Protocol 18 Transmission by Whiteflies (Aleyrodidae) --; Protocol 19 Transmission by Thrips (Thripidae) --; Exercise 13 Wedge-Grafting of Tobacco --; Exercise 14 Separation of a Nonpersistently and a Persistently Transmitted Virus by Differential Transmission --; Exercise 15 Visualisation of Salivary Sheaths Formed by Aphids --; Exercise 16 Separation of a Semipersistently Transmitted and a Circulative-Propagative Virus by Differential Transmission --; VI Maintenance of Virus Isolates --; Protocol 20 Freezing --; Protocol 21 Dehydration --; Protocol 22 Freeze-Drying --; Protocol 23 Short-Term Storage --; VII Production of Virus-Free Material from Infected Plants --; Protocol 24 Heat Treatment --; Protocol 25 Meristem-Tip Culture --; Protocol 26 Heat Treatment Combined with Meristem-Tip Culture --; VIII Virus Isolation and Purification --; Protocol 27 Density-Gradient Centrifugation --; Protocol 28 Virus Purification Protocols --; IX Physicochemical Properties of Virus Particle --; Protocol 29 UV Light Absorption --; Protocol 30 Buoyant Density in Caesium Chloride --; Protocol 31 Particle Morphology and Size as Determined with the Electron Microscope --; X Properties of Viral Components --; Protocol 32 Determination of the Molecular Mass of Coat Proteins by Gel Electrophoresis --; Protocol 33 RNA Extraction from Purified Virus Particles --; Protocol 34 Determination of the Molecular Mass of Ribonucleic Acids by Gel Electrophoresis --; XI Serological Techniques for Detection and Identification of Viruses and Viral Products --; Protocol 35 Production of Antiserum --; Protocol 36 Absorption of Antiserum --; Protocol 37 Chloroplast Agglutination Test --; Protocol 38 Microprecipitin Test --; Protocol 39 Gel Double-Diffusion Test in Plates --; Protocol 40 Enzyme-Linked Immunosorbent Assay --; Protocol 41 Immunoblotting Assays --; Exercise 17 Standard DAS-ELISA --; XII Electron Microscope and Light Microscope Serology --; Protocol 42 Electron Microscope Serology --; Protocol 43 Light Microscope Serology --; XIII Detection of Viral RNA --; Protocol 44 Northern Blotting --; Protocol 45 Northern Hybridisation --; Protocol 46 Dot-Blot Hybridisation (Nucleic Acid Spot Hybridisation) --; Protocol 47 Polymerase Chain Reaction --; XIV Diagnosis of a Disease and Identification of the Causal Virus(es) --; XIV Diagnosis of a Disease and Identification of the Causal Virus(es).

Unlike most pathogenic organisms, viruses require a special approach to establish their presence in a diseased plant, since they are not visible, even with a light microscope. Due to the tremendous damage of crop plants caused by viruses various methods and techniques have been developed to enable the investigation of these pathogens. A variety of protocols for determining the properties and identity of a virus or its behaviour in an infected plant are described in this manual. For teaching purposes, a number of class exercises have been added. The protocols have been selected in such a way as to be useful for plant viruses in both tropical and non-tropical parts of the world.

Agriculture.

Forests and forestry.

Life sciences.

by Jeanne Dijkstra, Cees P. Jager.

Cees P Jager

Jeanne Dijkstra

[Book]

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