عنوان

Plant transposable elements

9781627035675 )alk. paper(

Plant transposable elements

edited by Thomas Peterson, Department of Agronomy, Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA

xv, 324 pages : illustrations )some color(

Methods in molecular biology,1064-3745 ;1057

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1057.1064-3745

Series: Springer protocols )Series(.1949-2448

"Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events." --Publisher website.

Historical overview of transposable element research /P.A. Peterson --Distinguishing variable phenotypes from variegation caused by transposon activities /V. Walbot --Using transposons for genetic mosaic analysis of plant development /P.W. Becraft --Survey of natural and transgenic gene markers used to monitor transposon activity /L. Krishnaswamy and T. Peterson --Molecular biology of maize Ac/Ds elements: An overview /K. Lazarow, M.L. Doll and R. Kunze --Gene tagging with engineered Ds elements in maize /Y. Li, G. Segal, Q. Wangm H.K. Donner --Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice /Y.H. Xuan ]and nine others[ --Isolation of sequences flanking Ac insertion sites by Ac casting /D. Wang and T. Peterson --Regulation of the mutator system of transposons in maize /D. Lisch --Using MuDR/Mu transposons in directed tagging strategies /V. Walbot and J. Questa --Genetic and molecular analyses of UniformMu transposon insertion lines /D.R. McCarty, M. Suzuki, C. Hunter, J. Collins, W.T. Avigne, K.E. Koch --Digestion-ligation-amplification )DLA(: A simple genome walking method to amplify unknown sequences flanking mutator )Mu( transposons and thereby facilitate gene cloning /S. Liu, A.P. Hsia and P.S. Schnable --Molecular genetics and epigenetics of CACTA elements /N.V. Fedoroff --Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in arabidopsis /A. Harb and A. Pereira --Reverse genetics in rice using Tos17 /D. Mieulet, A. Dievart, G. Droc, N. Lanau, E. Guiderdoni --Identification and applications of the Petunia Class II Act1/dTph1 transposable element system /T. Gerats, J. Zethof and M. Vandenbussche --Transposon display: A versatile method for transposon tagging /M. Vandenbussche, J. Zethof and T. Gerats --Massive indexed parallel identification of transposon flanking sequences /M. Vandenbussche, J. Zethof and T. Gerats --Use of next generation sequencing )NGS( technologies for the genome-wide detection of transposition /M. Elbaidouri, C. Chaparro and O. Panaud --Overview of repeat annotation and de novo repeat identification /N. Jiang --Computational methods for identification of DNA transposons /N. Jiang --Tenest 2.0: Computational annotation and visualization of nested transposable elements /B.A. Kronmiller and R.P. Wise.

Includes bibliographical references and index.

methods and protocols

Transposons^aLaboratory manuals

Plant genetic transformation

DNA Transposable Elements^aLaboratory Manuals

Genome, Plant^agenetics--Laboratory Manuals

Laboratory Manuals.^amesh

Plant genetic transformation.^afast

Transposons.^afast

Handbooks, manuals, etc.^afast

Peterson, Thoma

1955

English Book