عنوان

Western blotting

9781493926930)alk. paper(

Western blotting

edited by Biji T. Kurien, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA, Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA, R. Hal Scofield, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA, Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA

New York

Springer

xiv, 509 pages : illustrations )chiefly color( ;

Methods molecular biology,1064-3745 ;1312

Springer protocols

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1312.1064-3745

Series: Springer protocols )Series(1949-2448

Early days of blotting /Edwin Southern --Origins of protein blotting /Harry Towbin --Western blotting : remembrance of things past /W. Neal Burnette --Simian virus 40 and protein transfer /Jaime Renart --Western blotting : an introduction /Biji T. Kurien and R. Hal Scofield --From little helpers to automation /Biji T. Kurien and Harry Towbin --Spectrophotometric methods to determine protein concentration /J.P. Dean Goldring --Solubilization of proteins : the importance of lysis buffer choice /Mandy Peach ]and three others[ --Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis /Der-Yen Lee and Geen-Dong Chang --Diffusion blotting : a rapid and simple method for production of multiple blots from a single gel /Ingrid Olsen and Harald G. Wiker --Multiple immunoblots by passive diffusion of proteins from a single SDS-PAGE gel /Biji T. Kurien and R. Hal Scofield --Slice blotting /Graeme Lowe --Localizing proteins by tissue printing /Rafael F. Pont-Lezica --Dot-immunobinding assay )Dot-Iba( /Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan --Analysis of antibody clonotype by affinity immunoblotting /Biji T. Kurien and R. Hal Scofield --Glycosaminoglycan blotting and detection after electrophoresis separation /Nicola Volpi and Francesca Maccari --Well-based reverse-phase protein array of formalin- fixed paraffin-embedded tissue /Joon-Yong Chung and Stephen M. Hewitt --Quantitative computerized western blotting in detail /Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth --Cationic electrophoresis and eastern blotting /Engelbert Buxbaum --Miniaturized blotting system for simultaneous detection of different autoantibodies /Ulrich Canzler ]and eight others[ --Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting /Joon-Yong Chungand Stephen M. Hewitt --Post-staining electroblotting for efficient and reliable peptide blotting /Der-Yen Lee and Geen-Dong Chang --Multistrip western blotting : a tool for comparative quantitative analysis of multiple proteins /Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin --Western blotting using PVDF membranes and its downstream applications /Setsuko Komatsu --Blotting from phastGel to membranes by ultrasound /Joseph Kost and Aharon Azagury --Western blotting of high and low molecular weight proteins using heat /Biji T. Kurien and R. Hal Scofield --Membrane strip affinity purification of autoantibodies /Biji T. Kurien --Strip immunoblotting of multiple antigenic peptides /Biji T. Kurien --Double-blotting : a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures /Francoise Lasne --Method for resolution and western blotting of very large proteins using agarose electrophoresis /Marion L. Greaser and Chad M. Warren --Immunodetection of P-selectin using an antibody to its C-terminal tag /Padmaja Mehta-D'souza --Improvements and variants of the multiple antigen blot assay-MABA : an immunoenzymatic technique for simultaneous antigen and antibody screening /Oscar Noya ]and six others[ --Blotting from immobilized pH gradient gels : application to total cell lysates /Harry Towbin --Immunoprecipitation : western blot for proteins of low abundance /Edward P. Trieu and Ira N. Targoff --Native electrophoresis and western blot analysis )NEWeB( : methods and applications /Ioannis N. Manoussopoulos and Mina Tsagris --Shift-western blotting : separate analysis of protein and DNA from protein-DNA complexes /Matthias Harbers --Grid-immunoblotting /Olga Yeliosof and R. Hal Scofield --Detection and quantification of protein-protein interactions by far-western blotting /Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida --Western blot analysis of adhesive interactions under fluid shear conditions : the blot rolling assay /Robert Sackstein and Robert Fuhlbrigge --Centrifuge blotting /Jinny Paul --Blotting of coomassie blue-stained proteins from PAGE gels to transparencies /Jinny Paul --B-cell ELISPOT : for the identification of antigen-specific antibody-secreting cells /Hemangi B. Shah and Kristi A. Koelsch --T cell ELISPOT : for the identification of specific cytokine-secreting T cells /Kerry M. Leehan and Kristi A. Koelsch --Membrane microplates for one- and two-color ELISPOT and FLUOROSPOT assays /Alexander E. Kalyuzhny --SDS-PAGE to immunoblot in one hour /Biji T. Kurien ]and three others[ --Single-cell western blotting /Syed M.S. Quadri --Protein detection by simple western analysis /Valerie M. Harris --Western blotting using microfluidics /Pothur R. Srinivas --Two-dimensional gel-based protein standardization verified by western blot analysis /Hisao Haniu ]and three others[ --Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase /Biji T. Kurien, Debashish Danda, and R. Hal Scofield --Other notable protein blotting methods : a brief review /Biji T. Kurien and R. Hal Scofield.

Includes bibliographical references and index.

methods and protocols

Western immunoblotting^aLaboratory manuals

Blotting, Western^amethods

Laboratory Manuals

Western immunoblotting.^afast--)OCoLC(fst01174036

Laboratory manuals.^afast--)OCoLC(fst01920776

Kurien, Biji T.

Scofield, R. Hal

English Book