عنوان

PCR primer design

9781493923649hardback : acid-free paper

PCR primer design

edited by Chhandak Basu, Department of Biology, California State University, Northridge, Los Angeles, CA, USA

Second edition.

New York

Springer

xii, 216 pages : illustrations )some color( ; 27 cm.

Methods in molecular biology,1064-3745 ;1275

Springer protocols,1949-2448

Series: Methods in molecular biology )Clifton, N.J.( ;1275,1064-3745

Series: Springer protocols )Series(,1949-2448

"This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." --Provided by publisher.

Fast masking of repeated primer binding sites in eukaryotic genomes / Reidar Andreson, Lauris Kaplinksi, and Maido Remm -- Primer design for PCR reactions in forensic biology / Kelly M. Elkins -- Design of primers and probes for quantitative real-time PCR methods / Alicia Rodraiguez, Mar Rodraiguez, Juan J. Caordoba, and Maraia J. Andrade -- Large-scale neucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus / Alexander Nagy, Tomaaes Jierinec, Lenka eCernaikovaa, Helena Jierincovaa, and Martina Havlaieckovaa -- Low-concentration initiator primers improve the amplification of gene targets with high sequence variability / Kenneth E. Pierce and Lawrence J. Wangh -- Multiplex PCR primer design for simultaneousu detection of multiple pathogens / Wenchao Yan -- Degenerate primer design for highly variable genomes / Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell -- Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection / Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu -- MultiPLX: automatic grouping and evaluation of PCR primers / Lauris Kaplinski and Maido Remm -- In silico PCR primer designing and validation / Anil Kumar and Nikita Chrodia -- Primer design using Primer Expressش for SYBR green-based quantitative PCR / Amarjeet Singh and Girdhar K. Pandey -- Designing primers for SNaPshot technique / Greciane Gaburro Paneto and Francisco de Paula Careta -- Rapid and simple method of qPCR primer design / Brenda Thornton and Chhandak Basu -- PRIMEGENSw3: a web-based tool for high-throughput primer and probe design / Garima Kushwaha, Gyan Prakash Srivastava, and Dong Xu -- Selecting specific PCR primers with MFEprimer / Wubin Qu and Chenggang Zhang.

Includes bibliographical references and index.

Polymerase chain reaction

Gene amplification

Gene amplification.^afast--)OCoLC(fst00939610

Polymerase chain reaction.^afast--)OCoLC(fst01070553

Basu, Chhandak

English Book