Series: Methods in molecular biology )Clifton, N.J.( ;v. 1296.1064-3745
Series: Springer protocols )Series(1949-2448
Small non-coding RNAs : a quick look in the rearview mirror /Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff --Alcoholic precipitation of small non-coding RNAs /Guillaume Clerget, Valaerie Bourguignon-Igel, and Mathieu Rederstorff --Quantification and quality control of a small non-coding RNA preparation /Virginie Marchand and Christiane Branlant --Impact of RNA isolation protocols on RNA detection by Northern blotting /Katrin Damm ]and seven others[ --Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes /Katrin Damm ]and seven others[ --Direct cloning of double-stranded RNAs /Manli Shen ]and three others[ --Detection and labeling of small non-coding RNAs by splinted ligation /Gabrielle Bourgeois ]and three others[ --Fluorescence in situ hybridization of small non-coding RNAs /Valentin Vautrot ]and three others[ --RT-qPCR-based quantification of small non-coding RNAs /Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele --Stem-loop RT-PCR based quantification of small non-coding RNAs /Vaeronique Salone and Mathieu Rederstorff --miR-RACE : an effective approach to accurately determine the sequence of computationally identified miRNAs /Chen Wang and Jinggui Fang --Probing small non-coding RNAs structures /Jean-Vincent Philippe ]and three others[ --cDNA library generation for the analysis of small RNAs by high-throughput sequencing /Jennifer Gebetsberger, Roger Fricker, and Norbert Polacek --CLIP-Seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells /Jaeraome Sauliaere and Hervae Le Hir --Microarray analysis of small non-coding RNAs /Michael Karbiener and Marcel Scheideler --RML-RACE, PPM-RACE, and qRT-PCR : an integrated strategy to accurately validate miRNA target genes /Chen Wang and Jinggui Fang --Dual luciferase gene reporter assays to study miRNA function /Thomas Claement, Vaeronique Salone, and Mathieu Rederstorff --Gene expression knockdown by transfection of siRNAs into mammalian cells /Yoann Abel and Mathieu Rederstorff --Efficient and selective knockdown of small non-coding RNAs /Xue-Hai Liang, Wen Shen, and Stanley T. Crooke --Cell-SELEX : in vitro selection of synthetic small specific ligands /Helena Dickinson ]and three others[ --Small non-coding RNAs and aptamers in diagnostics and therapeutics /Marissa Leonard, Yijuan Zhang, and Xiaoting Zhang.
Includes bibliographical references and index.
methods and protocols
Non-coding RNA^aLaboratory manuals
RNA, Small Untranslated^agenetics--Laboratory Manuals
RNA, Small Untranslated^aphysiology--Laboratory Manuals