عنوان

Protein-protein interactions

9781493924240 )alk. paper(

Protein-protein interactions

edited by Cheryl L. Meyerkord, Department of Pharmacology and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA, USA; Haian Fu, Department of Pharmacology, Department of Hematology & Medical Oncology, Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA, USA

Second edition.

xv, 620 pages : illustrations )some color(

Methods in molecular biology,1064-3745 ;1278

Springer protocols,1949-2448

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1278.

Structural basis of protein-protein interactions -- Quantitative analysis of protein-protein interactions -- Protein-protein interaction databases -- Computational prediction of protein-protein interactions. -- Structure-based computational approaches for small-molecule modulation of protein-protein interactions -- Targeting protein-protein interactions for drug discovery -- Studying protein-protein interactions using surface plasmon resonance -- Resonant waveguide grating for monitoring bimolecular interactions -- Quartz microbalance technology for probing biomolecular interactions -- Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry -- Characterization of protein-protein interactions by isothermal titration calorimetry -- Sedimentation equilibrium studies -- Detecting protein-protein interactions by gel filtration chromatography -- Using light scattering to determine the stoichiometry of protein complexes -- Circular dichroism )CD( analyses of protein-protein interactions -- Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy -- Quantitative protein analysis by mass spectrometry -- Using peptide arrays created by the SPOT method for defining protein-protein interactions -- Fluorescence polarization assay to quantify protein-protein interactions -- Faeorster resonance energy transfer )FRET( microscopy for monitoring biomolecular interactions -- Utilizing ELISA to monitor protein-protein interactions -- Glutathione-S-transferase )GST(-fusion based assays for studying protein-protein interactions -- Hexahistidine )6xHis( fusion-based assays for protein-protein interactions -- Studying protein-protein interactions via blot overlay/Far Western blot -- Co-immunoprecipitation from transfected cells -- In vivo protein cross-linking -- Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening -- Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae -- MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions -- Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells -- Mapping biochemical networks with protein fragment complementation assays -- Detection of protein-protein interaction using bimolecular fluorescence complementation assay -- Split-luciferase complementation assay to detect channel-protein interactions in live cells -- Confocal microscopy for intracellular co-localization of proteins -- Fluorescence polarization assay to quantify protein-protein interactions in an HTS format -- Estrogen receptor alpha/co-activator interaction assay -- TR-FRET -- High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions -- Discovery of inhibitors of the MDM2-p53 protein-protein interaction -- Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions.

Includes bibliographical references and index.

methods and applications

Protein-protein interactions^aLaboratory manuals

Meyerkord, Cheryl L.

Fu, Haian

English Book