عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq2408273834
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Gene Duplication, Divergence, and What Lies in Between
نام عام مواد
[Thesis]
نام نخستين پديدآور
Morgenthaler, Andrew Bank
نام ساير پديدآوران
Copley, Shelley D
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Colorado at Boulder
تاریخ نشرو بخش و غیره
2020
مشخصات ظاهری
نام خاص و کميت اثر
223
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Colorado at Boulder
امتياز متن
2020
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Life has repeatedly evolved new functions throughout its history, allowing life to survive and diversify for 3.8 billion years. The evolution of a novel function frequently requires the evolution of a new gene that encodes for an enzyme with a new function. New enzymes often evolve by amplification and divergence of preexisting genes encoding enzymes with a weak ability to provide a new function. Previous experimental studies have followed the evolutionary trajectory of an amplified gene, but have not considered mutations elsewhere in the genome when fitness is limited by an evolving gene. I have evolved a strain of Escherichia coli in which a secondary promiscuous activity has been recruited to serve an essential function. The gene encoding the 'weak-link' enzyme amplified in all eight populations, but mutations improving the newly needed activity occurred in only one. Most adaptive mutations occurred elsewhere in the genome to improve flux through the bottlenecked pathway. Finally, I compared the evolution of a strain of Escherichia coli grown in two different selective conditions: one in which the original function of an evolving enzyme is no longer required for growth and one in which both the new and original functions are required for growth. While the amplified region of the genome surrounding the evolving gene differed between the two selective conditions, most of the adaptive mutations in the two conditions were similar, suggesting the new activity of the evolving enzyme is more rate-limiting for growth than its native activity. Together these results depict how an organism evolves during the evolution of a new enzymatic function.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Enzyme evolution
موضوع مستند نشده
Enzyme promiscuity
موضوع مستند نشده
Gene duplication and divergence
موضوع مستند نشده
Innovation-amplification-divergence
موضوع مستند نشده
Metabolic pathway evolution
موضوع مستند نشده
Molecular biology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Copley, Shelley D
مستند نام اشخاص تاييد نشده
Morgenthaler, Andrew Bank
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
