عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304192960
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Induction of bovine respiratory syncytial virus vaccinal immunity
نام عام مواد
[Thesis]
نام نخستين پديدآور
M. Zulfiqar
نام ساير پديدآوران
M. L. Kaeberle
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Iowa State University
تاریخ نشرو بخش و غیره
1995
مشخصات ظاهری
نام خاص و کميت اثر
158
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
Iowa State University
امتياز متن
1995
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Electron microscopic examination of ultrathin sections of BRSV strain 375-infected Vero cells revealed not only spherical and filamentous forms of the virus but also bridges between the spherical particles. The bridges did not appear to be arranged at a particular angle. Because of the bridges the virus particles occurred in large aggregates requiring that this factor be taken into account while purifying the virus. Most of the observed mature particles were extracellular. However a few spherical particles with and without internal structure were also present in intracytoplasmic vesicles. A panel of monoclonal antibodies (MAbs), derived from the fusing of the primed BALB/C mice splenocytes to SP2/O cells, were generated against the N protein of BRSV. The MAbs were characterized by ELISA, a radioimmunoprecipitation assay, indirect immunofluorescence, and epitope mapping. According to the class and subclass of Ig these MAbs can be grouped into four groups. Eight MAbs, two from each group, were used for epitope mapping. The reaction pattern of the MAbs indicated 4 to 6 antigenic sites on the N protein. Two monoclonal antibodies 65-12 (IgM) and 38-6 (IgG2a) were used to make solid matrix-antibody-antigen complexes (SMAA). The SMAA complexes so formed were washed five times with PBS, suspended in an equal quantity of aluminum hydroxide gel and used for immunization of two groups of lambs. In immunized groups the maximum immune response in terms of lymphocyte blastogenic responses was observed on day 11 postvaccination. However, the response of animals in group B, vaccinated with SMAA complexes containing MAb (IgM) was significantly higher than the group C, vaccinated with SMAA complexes containing MAb (IgG2a). The group B animals also exhibited a marked humoral response to N protein in western immunoblot assay as compared to group C and control animals 11 days postvaccination. None of the animals developed a SN antibody titer after vaccination. Subsequent challenging, 21 days postvaccination, with heat inactivated BRSV resulted in a far better SN titer in the SMAA complexes vaccinated groups than the control group. Not only did vaccinated animals developed a higher titer but the response developed earlier since all vaccinated animals seroconverted within five days of challenge.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Veterinary services
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
M. L. Kaeberle
مستند نام اشخاص تاييد نشده
M. Zulfiqar
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
