عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304376344
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Immunohistochemical analysis of cytochrome P450 isozymes (CYP2B1 & CYP1A1) and NADPH-cytochrome P450 reductase during lung tumour development in SWR/J mice
نام عام مواد
[Thesis]
نام نخستين پديدآور
J. A. Lord
نام ساير پديدآوران
P. G. Forhert
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Queen's University (Canada)
تاریخ نشرو بخش و غیره
1997
مشخصات ظاهری
نام خاص و کميت اثر
86
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.Sc.
کسي که مدرک را اعطا کرده
Queen's University (Canada)
امتياز متن
1997
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
The cytochrome P450 enzymes represent a family of hemoproteins, which together with the flavoprotein NADPH-cytochrome P450 reductase, are responsible for the oxidative metabolism of multiple xenobiotics and endogenous compounds. These enzymes are classified according to proposed evolutionary relationships. As such, an italicized root symbol CYP denotes the cytochrome P450 gene (Cyp for mouse), an Arabic number identifies the family, a letter distinguishes the subfamily and an Arabic numeral denotes the individual gene. Gene products include only capital letters, without italics. Previous studies have postulated that murine lung tumourigenesis may be correlated with decreased cytochrome P450 expression. The present study utilized polyclonal antibodies and avidin-biotin horseradish peroxidase, to immunohistochemically detect CYP2B1, CYP1A1 and NADPH-cytochrome P450 reductase in developing tumours and non-neoplastic tissues within SWR/J murine lung. Mice were administered the procarcinogen urethane (1 mg/g) and sacrificed at designated stages of neoplastic development. Developing tumours were categorized as hyperplastic foci (6-10 weeks), solid or papillary adenomas (16-22 weeks) and solid or papillary carcinomas (52 weeks). To induce CYP1A1, mice were administered usd\betausd-naphthoflavone (usd\betausd-NF), in corn oil (80 mg/kg) prior to sacrifice. Control mice received only the vehicle. In control mice, CYP2B1 was localized in Clara and type II cells, while CYP1A1 was not immunohistochemically detected. Following treatment with usd\betausd-NF, CYP2B1 localization was not altered; however, CYP1A1 was detected in endothelial and type II cells. NADPH-cytochrome P450 reductase was detected in Clara and type II cells of all mice. CYP2B1 was weakly detected in all developing tumours. In usd\betausd-NF-treated mice, CYP1A1 and NADPH-cytochrome P350 reductase were detected in hyperplastic foci, solid adenomas and to a lesser extent in papillary adenomas, but were absent in carcinomas. Furthermore, localization of both CYP1A1 and NADPH-cytochrome P450 reductase was most pronounced in hyperplastic foci within carcinoma-bearing lungs. These results suggest that tumour formation is associated with diminished expression of CYP2B1, CYP1A1, and NADPH-cytochrome P450 reductase.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Cellular biology
موضوع مستند نشده
Cellular biology
موضوع مستند نشده
Health and environmental sciences
موضوع مستند نشده
Molecular biology
موضوع مستند نشده
Oncology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
J. A. Lord
مستند نام اشخاص تاييد نشده
P. G. Forhert
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
